Frontiers in Pharmacology (Aug 2022)
Ameliorative effect and mechanism of Si-Ni-San on chronic stress-induced diarrhea-irritable bowel syndrome in rats
- Hui-Yu Chen,
- Hui-Yu Chen,
- Hui-Yu Chen,
- Hui-Yu Chen,
- Hui-Yu Chen,
- Hui-Yu Chen,
- Hui-Yu Chen,
- Jian Liu,
- Jian Liu,
- Jian Liu,
- Jian Liu,
- Jian Liu,
- Jian Liu,
- Ding-Zhou Weng,
- Ding-Zhou Weng,
- Ding-Zhou Weng,
- Ding-Zhou Weng,
- Ding-Zhou Weng,
- Ding-Zhou Weng,
- Ding-Zhou Weng,
- Li Yan,
- Li Yan,
- Li Yan,
- Li Yan,
- Li Yan,
- Li Yan,
- Chun-Shui Pan,
- Chun-Shui Pan,
- Chun-Shui Pan,
- Chun-Shui Pan,
- Chun-Shui Pan,
- Chun-Shui Pan,
- Kai Sun,
- Kai Sun,
- Kai Sun,
- Kai Sun,
- Kai Sun,
- Kai Sun,
- Xiao Guo,
- Xiao Guo,
- Xiao Guo,
- Xiao Guo,
- Xiao Guo,
- Xiao Guo,
- Di Wang,
- Di Wang,
- Di Wang,
- Di Wang,
- Di Wang,
- Di Wang,
- Di Wang,
- Gulinigaer Anwaier,
- Gulinigaer Anwaier,
- Gulinigaer Anwaier,
- Gulinigaer Anwaier,
- Gulinigaer Anwaier,
- Gulinigaer Anwaier,
- Gulinigaer Anwaier,
- Ying-Qian Jiao,
- Ying-Qian Jiao,
- Ying-Qian Jiao,
- Ying-Qian Jiao,
- Ying-Qian Jiao,
- Ying-Qian Jiao,
- Ying-Qian Jiao,
- Zhi-Xin Li,
- Zhi-Xin Li,
- Zhi-Xin Li,
- Zhi-Xin Li,
- Zhi-Xin Li,
- Zhi-Xin Li,
- Jing-Yan Han,
- Jing-Yan Han,
- Jing-Yan Han,
- Jing-Yan Han,
- Jing-Yan Han,
- Jing-Yan Han,
- Jing-Yan Han
Affiliations
- Hui-Yu Chen
- Department of Integration of Chinese and Western Medicine, School of Basic Medical Sciences, Peking University, Beijing, China
- Hui-Yu Chen
- Tasly Microcirculation Research Center, Peking University Health Science Center, Beijing, China
- Hui-Yu Chen
- Academy of Integration of Chinese and Western Medicine, Peking University Health Science Center, Beijing, China
- Hui-Yu Chen
- Key Laboratory of Microcirculation, State Administration of Traditional Chinese Medicine of the People’s Republic of China, Beijing, China
- Hui-Yu Chen
- Key Laboratory of Stasis and Phlegm, State Administration of Traditional Chinese Medicine of the People’s Republic of China, Beijing, China
- Hui-Yu Chen
- State Key Laboratory of Core Technology in Innovative Chinese Medicine, Tianjin, China
- Hui-Yu Chen
- Beijing Microvascular Institute of Integration of Chinese and Western Medicine, Beijing, China
- Jian Liu
- Department of Integration of Chinese and Western Medicine, School of Basic Medical Sciences, Peking University, Beijing, China
- Jian Liu
- Academy of Integration of Chinese and Western Medicine, Peking University Health Science Center, Beijing, China
- Jian Liu
- Key Laboratory of Microcirculation, State Administration of Traditional Chinese Medicine of the People’s Republic of China, Beijing, China
- Jian Liu
- Key Laboratory of Stasis and Phlegm, State Administration of Traditional Chinese Medicine of the People’s Republic of China, Beijing, China
- Jian Liu
- State Key Laboratory of Core Technology in Innovative Chinese Medicine, Tianjin, China
- Jian Liu
- Beijing Microvascular Institute of Integration of Chinese and Western Medicine, Beijing, China
- Ding-Zhou Weng
- Department of Integration of Chinese and Western Medicine, School of Basic Medical Sciences, Peking University, Beijing, China
- Ding-Zhou Weng
- Tasly Microcirculation Research Center, Peking University Health Science Center, Beijing, China
- Ding-Zhou Weng
- Academy of Integration of Chinese and Western Medicine, Peking University Health Science Center, Beijing, China
- Ding-Zhou Weng
- Key Laboratory of Microcirculation, State Administration of Traditional Chinese Medicine of the People’s Republic of China, Beijing, China
- Ding-Zhou Weng
- Key Laboratory of Stasis and Phlegm, State Administration of Traditional Chinese Medicine of the People’s Republic of China, Beijing, China
- Ding-Zhou Weng
- State Key Laboratory of Core Technology in Innovative Chinese Medicine, Tianjin, China
- Ding-Zhou Weng
- Beijing Microvascular Institute of Integration of Chinese and Western Medicine, Beijing, China
- Li Yan
- Tasly Microcirculation Research Center, Peking University Health Science Center, Beijing, China
- Li Yan
- Academy of Integration of Chinese and Western Medicine, Peking University Health Science Center, Beijing, China
- Li Yan
- Key Laboratory of Microcirculation, State Administration of Traditional Chinese Medicine of the People’s Republic of China, Beijing, China
- Li Yan
- Key Laboratory of Stasis and Phlegm, State Administration of Traditional Chinese Medicine of the People’s Republic of China, Beijing, China
- Li Yan
- State Key Laboratory of Core Technology in Innovative Chinese Medicine, Tianjin, China
- Li Yan
- Beijing Microvascular Institute of Integration of Chinese and Western Medicine, Beijing, China
- Chun-Shui Pan
- Tasly Microcirculation Research Center, Peking University Health Science Center, Beijing, China
- Chun-Shui Pan
- Academy of Integration of Chinese and Western Medicine, Peking University Health Science Center, Beijing, China
- Chun-Shui Pan
- Key Laboratory of Microcirculation, State Administration of Traditional Chinese Medicine of the People’s Republic of China, Beijing, China
- Chun-Shui Pan
- Key Laboratory of Stasis and Phlegm, State Administration of Traditional Chinese Medicine of the People’s Republic of China, Beijing, China
- Chun-Shui Pan
- State Key Laboratory of Core Technology in Innovative Chinese Medicine, Tianjin, China
- Chun-Shui Pan
- Beijing Microvascular Institute of Integration of Chinese and Western Medicine, Beijing, China
- Kai Sun
- Tasly Microcirculation Research Center, Peking University Health Science Center, Beijing, China
- Kai Sun
- Academy of Integration of Chinese and Western Medicine, Peking University Health Science Center, Beijing, China
- Kai Sun
- Key Laboratory of Microcirculation, State Administration of Traditional Chinese Medicine of the People’s Republic of China, Beijing, China
- Kai Sun
- Key Laboratory of Stasis and Phlegm, State Administration of Traditional Chinese Medicine of the People’s Republic of China, Beijing, China
- Kai Sun
- State Key Laboratory of Core Technology in Innovative Chinese Medicine, Tianjin, China
- Kai Sun
- Beijing Microvascular Institute of Integration of Chinese and Western Medicine, Beijing, China
- Xiao Guo
- Department of Integration of Chinese and Western Medicine, School of Basic Medical Sciences, Peking University, Beijing, China
- Xiao Guo
- Academy of Integration of Chinese and Western Medicine, Peking University Health Science Center, Beijing, China
- Xiao Guo
- Key Laboratory of Microcirculation, State Administration of Traditional Chinese Medicine of the People’s Republic of China, Beijing, China
- Xiao Guo
- Key Laboratory of Stasis and Phlegm, State Administration of Traditional Chinese Medicine of the People’s Republic of China, Beijing, China
- Xiao Guo
- State Key Laboratory of Core Technology in Innovative Chinese Medicine, Tianjin, China
- Xiao Guo
- Beijing Microvascular Institute of Integration of Chinese and Western Medicine, Beijing, China
- Di Wang
- Department of Integration of Chinese and Western Medicine, School of Basic Medical Sciences, Peking University, Beijing, China
- Di Wang
- Tasly Microcirculation Research Center, Peking University Health Science Center, Beijing, China
- Di Wang
- Academy of Integration of Chinese and Western Medicine, Peking University Health Science Center, Beijing, China
- Di Wang
- Key Laboratory of Microcirculation, State Administration of Traditional Chinese Medicine of the People’s Republic of China, Beijing, China
- Di Wang
- Key Laboratory of Stasis and Phlegm, State Administration of Traditional Chinese Medicine of the People’s Republic of China, Beijing, China
- Di Wang
- State Key Laboratory of Core Technology in Innovative Chinese Medicine, Tianjin, China
- Di Wang
- Beijing Microvascular Institute of Integration of Chinese and Western Medicine, Beijing, China
- Gulinigaer Anwaier
- Department of Integration of Chinese and Western Medicine, School of Basic Medical Sciences, Peking University, Beijing, China
- Gulinigaer Anwaier
- Tasly Microcirculation Research Center, Peking University Health Science Center, Beijing, China
- Gulinigaer Anwaier
- Academy of Integration of Chinese and Western Medicine, Peking University Health Science Center, Beijing, China
- Gulinigaer Anwaier
- Key Laboratory of Microcirculation, State Administration of Traditional Chinese Medicine of the People’s Republic of China, Beijing, China
- Gulinigaer Anwaier
- Key Laboratory of Stasis and Phlegm, State Administration of Traditional Chinese Medicine of the People’s Republic of China, Beijing, China
- Gulinigaer Anwaier
- State Key Laboratory of Core Technology in Innovative Chinese Medicine, Tianjin, China
- Gulinigaer Anwaier
- Beijing Microvascular Institute of Integration of Chinese and Western Medicine, Beijing, China
- Ying-Qian Jiao
- Department of Integration of Chinese and Western Medicine, School of Basic Medical Sciences, Peking University, Beijing, China
- Ying-Qian Jiao
- Tasly Microcirculation Research Center, Peking University Health Science Center, Beijing, China
- Ying-Qian Jiao
- Academy of Integration of Chinese and Western Medicine, Peking University Health Science Center, Beijing, China
- Ying-Qian Jiao
- Key Laboratory of Microcirculation, State Administration of Traditional Chinese Medicine of the People’s Republic of China, Beijing, China
- Ying-Qian Jiao
- Key Laboratory of Stasis and Phlegm, State Administration of Traditional Chinese Medicine of the People’s Republic of China, Beijing, China
- Ying-Qian Jiao
- State Key Laboratory of Core Technology in Innovative Chinese Medicine, Tianjin, China
- Ying-Qian Jiao
- Beijing Microvascular Institute of Integration of Chinese and Western Medicine, Beijing, China
- Zhi-Xin Li
- Department of Integration of Chinese and Western Medicine, School of Basic Medical Sciences, Peking University, Beijing, China
- Zhi-Xin Li
- Academy of Integration of Chinese and Western Medicine, Peking University Health Science Center, Beijing, China
- Zhi-Xin Li
- Key Laboratory of Microcirculation, State Administration of Traditional Chinese Medicine of the People’s Republic of China, Beijing, China
- Zhi-Xin Li
- Key Laboratory of Stasis and Phlegm, State Administration of Traditional Chinese Medicine of the People’s Republic of China, Beijing, China
- Zhi-Xin Li
- State Key Laboratory of Core Technology in Innovative Chinese Medicine, Tianjin, China
- Zhi-Xin Li
- Beijing Microvascular Institute of Integration of Chinese and Western Medicine, Beijing, China
- Jing-Yan Han
- Department of Integration of Chinese and Western Medicine, School of Basic Medical Sciences, Peking University, Beijing, China
- Jing-Yan Han
- Tasly Microcirculation Research Center, Peking University Health Science Center, Beijing, China
- Jing-Yan Han
- Academy of Integration of Chinese and Western Medicine, Peking University Health Science Center, Beijing, China
- Jing-Yan Han
- Key Laboratory of Microcirculation, State Administration of Traditional Chinese Medicine of the People’s Republic of China, Beijing, China
- Jing-Yan Han
- Key Laboratory of Stasis and Phlegm, State Administration of Traditional Chinese Medicine of the People’s Republic of China, Beijing, China
- Jing-Yan Han
- State Key Laboratory of Core Technology in Innovative Chinese Medicine, Tianjin, China
- Jing-Yan Han
- Beijing Microvascular Institute of Integration of Chinese and Western Medicine, Beijing, China
- DOI
- https://doi.org/10.3389/fphar.2022.940463
- Journal volume & issue
-
Vol. 13
Abstract
Background: Chronic stress-induced diarrhea is a common clinical condition, characterized by an abnormal bowel movement and loose stools, which lacks effective treatment in the clinic. Si-Ni-San (SNS) is a compound traditional Chinese medicine extensively used in China for stress-related diarrhea. However, the mechanism is unclear.Methods: Male Wistar rats (200 ± 20 g) were placed in a restraint cylinder and fixed horizontally for 3 h once daily for 21 consecutive days to establish a chronic restraint stress (CRS) rat model. SNS (0.6944 g/kg or 1.3888 g/kg) was given by gavage 1 h before the restraint once daily for 21 consecutive days. We examined the fecal score, dopamine β hydroxylase (DβH), and c-fos expression in locus coeruleus, norepinephrine (NE) content in ileum and plasma, expression of α1 adrenergic receptors, MLCK, MLC, and p-MLC in the colon and mesenteric arteries, contraction of isolated mesenteric arteries, The expression of subunit δ of ATP synthase (ATP5D) in intestinal tissues, ATP, ADP, and AMP content in the ileum and colon, occludin expression between ileum epithelial cells, the number of enterochromaffin cells (ECs) and mast cells (MCs) in the ileum, and 5-hydroxytryptamine (5-HT) content in the ileum and plasma.Results: After SNS treatment, the fecal score was improved. The increased expression of DβH and c-fos in locus coeruleus was inhibited. SNS suppressed the increased NE content in the ileum and plasma, down-regulated α1 adrenergic receptors in mesenteric arteries and MLCK, MLC, p-MLC in the colon and mesenteric arteries, and inhibited the contraction of mesenteric arteries. SNS also increased the ATP content in the ileum and colon, inhibited low expression of ATP5D in intestinal tissues, inhibited the decrease of ATP/ADP in the ileum and ATP/AMP in the colon, and up-regulated the occludin expression between ileum epithelial cells. In addition, SNS inhibited the increase of ECs and MCs in the ileum and the increase of 5-HT content in the ileum and plasma.Conclusion: This study demonstrated that SNS could improve CRS-induced abnormal feces in rats. This effect was related to the inhibition of CRS-induced increased expression of DβH and c-fos in the locus coeruleus, NE content in the ileum and plasma, and the contraction of isolated mesenteric arteries; inhibition of energy metabolism abnormality and decreased occludin expression; inhibition of increased ECs and MCs in the ileum, and 5-HT content in the ileum and plasma.
Keywords
- Si-Ni-San
- sympathetic nerves excitation
- energy metabolism
- intestinal mucosal barrier
- tight junctions
