eJHaem (Nov 2022)

Protein profiling by reverse phase protein array (RPPA) in classical hairy cell leukemia (HCL) and HCL‐variant

  • Fieke W. Hoff,
  • Ti'ara L. Griffen,
  • Yihua Qiu,
  • Steven M. Kornblau

DOI
https://doi.org/10.1002/jha2.558
Journal volume & issue
Vol. 3, no. 4
pp. 1321 – 1325

Abstract

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Abstract Classical hairy cell leukemia (HCL‐c) and HCL variant (HCL‐v) are recognized as separate entities with HCL‐v having significantly shorter overall survival. Proteomic studies, shown to be prognostic in various forms of leukemia, have not been performed in HCL. We performed reverse phase protein array‐based protein profiling with 384 antibodies in HCL‐c (n = 12), HCL‐v (n = 4), and normal B‐cells (n = 5) samples. While HCL could be distinguished from normal based on unsupervised hierarchical clustering, overlap in protein expression patterns was seen between HCL‐c and HCL‐v, with ∼10% of the proteins being differentially expressed, suggesting potential therapeutic targets.

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