Detection and Prevalence of Herpes Simplex Virus and Papilloma Virus from Bladder Cancer Tissue by PCR from Doctor Gharazi Hospital, Malayer - Hamadan in 2014

Abstract

Background and Aim: Bladder cancer is the fourth most common cancer in men and the eighth form of cancer in women. Role of the viral infections risk has been much discussed in recent years. Therefore the aim of this study was to optimize a method based on polymerase chain reaction to detect and evaluate prevalence of herpes simplex virus and papilloma virus in bladder tumors. Materials and Methods: forty cases of of the bladder TCC carcinoma in the paraffin from the pathology department of the Doctor Gharazi hospital, malayer were collected in the summer 2014. After deparaffin samples, DNA was extracted from the tissue and the beta-globin gene was amplification for the accuracy of the DNA extracted. The gene specific primers for HPV L1 protein and UL130 gene using by PCR for HSV. To examine the relationship between viruses and cancer incidence, was used chi-square test (X2). Results: From 40 Carcinoma samples studied 15 cases (47.39%) were men infected with HPV virus, None of the women surveyed have been infected with HPV. HSV wasn’t detected in 40 samples collected. Conclusions: Considering that independence validation of chi-square test equal to 0.664, and greater than 0.05, correlation between the incidence of HPV virus and no bladder tumor with 95% trust. We also compare the results with in other parts of the world, a lower incidence of HPV infection compared to some other countries were reported.

Keywords

• Bladder Carcinoma
• PCR
• HSV
• HPV