The Effect of Humic Acid on Plant Growth, Phytoremediation and Oxidative Stress in Rapeseed (Brassica napus L.) Grown Under Heavy Metal Stress

Abstract

The aim of this study was to investigate the effects of humic acid (HA) applications on rapeseed (Brassica napus L.) growth, heavy metal uptake, bioconcentration factor (BCF), translocation factor (TF), tolerance index (TI), catalase (CAT), ascorbate peroxidase (APX) enzyme activities and hydrogen peroxide (H2O2) content in polluted soil with lead (Pb), chromium (Cr), cadmium (Cd), and zinc (Zn). Three doses of HA (Control, HA1:500 mg kg-1, HA2:1000 mg kg-1, HA3:2000 mg kg-1) were applied in pots. HA1, HA2, and HA3 applications increased plant growth parameters compared to polluted soil. Compared to the control, HA applications in polluted soil increased the Pb, Cr, Cd, and Zn concentrations in the plant. However, HA applications in polluted soil significantly decreased the heavy metal content in roots and shoots of the plant compared to polluted soil. BCF in both roots and shoots of the plants were greater than 1 for Pb, Cr, Cd, and Zn. However, specifically HA2 application decreased the shoot and root BCF values in polluted soil. TF was smaller than 1 in Pb, Cr, Cd, and Zn in polluted soil. On the other hand, HA applications for Cd increased TF values. Shoot TI decreased 17.37 %, and root TI decreased 9.09% in polluted soil. CAT and APX enzyme activities and H2O2 increased significantly in polluted soil. However, HA applications decreased CAT and APX enzyme activities and H2O2 content in rapeseed. It is concluded that HA application in Pb, Cr, Cd, and Zn polluted soil has a remedial effect on the development of rapeseed by reducing heavy metal content and oxidative stress.

Keywords

• antioxidative activity
• heavy metal
• humic acid
• plant growth
• rapeseed (brassica napus l.)