Computer Assisted Semen Analysis of Epididymal Spermatozoa after an Interperitoneal Administration of Diazinon and Cadmium

Abstract

The effects of cadmium and diazinon administration on rat sperm motility parameters were performed using a Computer Assisted Semen Analyzer (CASA) system. CASA was made on semen samples collected 36 h following an intraperitoneal injection of physiological solution (control group), diazinon (20 mg/kg body wt), cadmium (2 mg/kg body wt), separately and in combination. Sperm kinematic measurements revealed significant decline in the percentage of motile spermatozoa (P<0.05), significant higher amplitude of lateral head displacement (ALH) (P<0.01) with significant decline in beat cross frequency (BCF) (P<0.001) after the diazinon administration. Cadmium-treated males exhibited significantly lower percentage of motile spermatozoa (P<0.01) and percentage of spermatozoa with progressive motility (P<0.001) with significant decrease in straightness (STR) and BCF (P<0.05). Simultaneous exposure to cadmium and diazinon led to significant increase in ALH (P<0.05) and significant decrease in BCF (P<0.001). Cadmium and diazinon exerted deleterious effect inducing spermatozoa motility alterations which could be subsequently negatively related to male fertility.

Keywords

• cadmium
• computer assisted semen analysis
• diazinon
• rat
• spermatozoa motility