Frontiers in Psychiatry (Aug 2020)
Sensitivity to Morphine Reward Associates With Gut Dysbiosis in Rats With Morphine-Induced Conditioned Place Preference
- Jingyuan Zhang,
- Jingyuan Zhang,
- Jingyuan Zhang,
- Jingyuan Zhang,
- Jingyuan Zhang,
- Jun Yang,
- Jun Yang,
- Jun Yang,
- Jun Yang,
- Jun Yang,
- Cheng Yang,
- Cheng Yang,
- Cheng Yang,
- Cheng Yang,
- Cheng Yang,
- Ti Chen,
- Ziwei Wang,
- Ziwei Wang,
- Ziwei Wang,
- Ziwei Wang,
- Ziwei Wang,
- Junyi Li,
- Junyi Li,
- Junyi Li,
- Junyi Li,
- Junyi Li,
- Fanglin Qin,
- Fanglin Qin,
- Fanglin Qin,
- Fanglin Qin,
- Fanglin Qin,
- Qijian Deng,
- Qijian Deng,
- Qijian Deng,
- Qijian Deng,
- Qijian Deng,
- Xiaojie Zhang,
- Xiaojie Zhang,
- Xiaojie Zhang,
- Xiaojie Zhang,
- Xiaojie Zhang
Affiliations
- Jingyuan Zhang
- Department of Psychiatry, The Second Xiangya Hospital, Central South University, Changsha, China
- Jingyuan Zhang
- National Clinic Research Center for Mental Disorders, Changsha, China
- Jingyuan Zhang
- National Technology Institute on Mental Disorders, Changsha, China
- Jingyuan Zhang
- Hunan Key Laboratory of Psychiatry and Mental Health, Changsha, China
- Jingyuan Zhang
- Mental Health Institute of Central South University, Changsha, China
- Jun Yang
- Department of Psychiatry, The Second Xiangya Hospital, Central South University, Changsha, China
- Jun Yang
- National Clinic Research Center for Mental Disorders, Changsha, China
- Jun Yang
- National Technology Institute on Mental Disorders, Changsha, China
- Jun Yang
- Hunan Key Laboratory of Psychiatry and Mental Health, Changsha, China
- Jun Yang
- Mental Health Institute of Central South University, Changsha, China
- Cheng Yang
- Department of Psychiatry, The Second Xiangya Hospital, Central South University, Changsha, China
- Cheng Yang
- National Clinic Research Center for Mental Disorders, Changsha, China
- Cheng Yang
- National Technology Institute on Mental Disorders, Changsha, China
- Cheng Yang
- Hunan Key Laboratory of Psychiatry and Mental Health, Changsha, China
- Cheng Yang
- Mental Health Institute of Central South University, Changsha, China
- Ti Chen
- Clinical Laboratory, The Third Xiangya Hospital, Central South University, Changsha, China
- Ziwei Wang
- Department of Psychiatry, The Second Xiangya Hospital, Central South University, Changsha, China
- Ziwei Wang
- National Clinic Research Center for Mental Disorders, Changsha, China
- Ziwei Wang
- National Technology Institute on Mental Disorders, Changsha, China
- Ziwei Wang
- Hunan Key Laboratory of Psychiatry and Mental Health, Changsha, China
- Ziwei Wang
- Mental Health Institute of Central South University, Changsha, China
- Junyi Li
- Department of Psychiatry, The Second Xiangya Hospital, Central South University, Changsha, China
- Junyi Li
- National Clinic Research Center for Mental Disorders, Changsha, China
- Junyi Li
- National Technology Institute on Mental Disorders, Changsha, China
- Junyi Li
- Hunan Key Laboratory of Psychiatry and Mental Health, Changsha, China
- Junyi Li
- Mental Health Institute of Central South University, Changsha, China
- Fanglin Qin
- Department of Psychiatry, The Second Xiangya Hospital, Central South University, Changsha, China
- Fanglin Qin
- National Clinic Research Center for Mental Disorders, Changsha, China
- Fanglin Qin
- National Technology Institute on Mental Disorders, Changsha, China
- Fanglin Qin
- Hunan Key Laboratory of Psychiatry and Mental Health, Changsha, China
- Fanglin Qin
- Mental Health Institute of Central South University, Changsha, China
- Qijian Deng
- Department of Psychiatry, The Second Xiangya Hospital, Central South University, Changsha, China
- Qijian Deng
- National Clinic Research Center for Mental Disorders, Changsha, China
- Qijian Deng
- National Technology Institute on Mental Disorders, Changsha, China
- Qijian Deng
- Hunan Key Laboratory of Psychiatry and Mental Health, Changsha, China
- Qijian Deng
- Mental Health Institute of Central South University, Changsha, China
- Xiaojie Zhang
- Department of Psychiatry, The Second Xiangya Hospital, Central South University, Changsha, China
- Xiaojie Zhang
- National Clinic Research Center for Mental Disorders, Changsha, China
- Xiaojie Zhang
- National Technology Institute on Mental Disorders, Changsha, China
- Xiaojie Zhang
- Hunan Key Laboratory of Psychiatry and Mental Health, Changsha, China
- Xiaojie Zhang
- Mental Health Institute of Central South University, Changsha, China
- DOI
- https://doi.org/10.3389/fpsyt.2020.00631
- Journal volume & issue
-
Vol. 11
Abstract
Gut microbiota has been found to establish a bidirectional relationship with the central nervous system. Variations of the gut microbiota has been implicated in various mental disorders, including opioid use disorders. Morphine exposure has been repeatedly found to disrupt the gut microbiota, but association between the gut microbiota and the sensitivity to morphine reward remains unknown. In this study the conditioned place preference (CPP) paradigm was used for morphine-treated rats and saline-treated rats. After the CPP procedure, the morphine-treated rats were divided equally into the low and high CPP (L- and H-CPP) groups according to the CPP scores. We adopted 16S rRNA sequencing for the fecal bacterial communities at baseline and post-conditioning. By comparing the morphine-treated group with saline-treated group, we found alterations of microbial composition in the morphine-treated group, but no significant differences in alpha diversity. The L-CPP group and H-CPP group differed in microbial composition both before and after morphine treatment. The relative abundance of certain taxa was correlated to the CPP scores, such as Alloprevotella and Romboutsia. This study provides direct evidence that morphine exposure alters the composition of the gut microbiota in rats and that microbial alterations are correlated to the sensitivity to morphine reward. These findings may help develop novel therapeutic and preventive strategies for opioid use disorder.
Keywords
- morphine
- gut microbiota
- gut dysbiosis
- conditioned place preference
- sensitivity to morphine reward
- rat model
