MORPHOLOGICAL CHANGES IN THE TESTICLES WITH ACTION ON THE BODY RUBOMYCIN HYDROCHLORIDE

Abstract

It is known that toxic drugs include rubomycin (daunomycin) – an antitumor antibiotic with a pronounced cytostatic effect, which is widely used in the clinic. The use of this drug can lead to various disorders of the cardiovascular, respiratory, digestive, hematopoietic systems. In men, under the influence of rubomycin hydrochloride, the functioning of the reproductive system may deteriorate and lead to azoospermia. It should be noted that the morphometric structure of the testis when exposed to rubomycin hydrochloride has not been studied. The purpose of the research – to study morphological changes in testis under the influence of rubomycin hydrochloride. Object and methods. The complex of histological methods examined morphological changes in the testis of 30 laboratory white male rats, which were divided into 2 groups. The first group consisted of 14 intact animals that were under normal vivarium conditions, the 2 – consisted of 16 rats administered intraperitoneally with rubomycin hydrochloride at a dose of 30 mg/kg. After 30 days after the beginning of the experiment, the euthanasia of the experimental animals was performed by bloodletting in conditions of thiopental anesthesia. The cut pieces of the testis were fixed in a 10 % neutral formalin solution, and after appropriate ethyl alcohol of increasing concentration was poured in paraffin blocks. Histologic sections 5-7 μm thick after deparaffinization were stained with hematoxylin-eosin, for van Gizon, Mallory, Weigert, and toluidine blue. Histological micropreparations were examined using MBD-15 and Lumam-P8 microscopes. Results and discussion. Histological examination of testicular macropreparations of experimental animals treated with rubomycin hydrochloride revealed pronounced structural changes. Under these experimental conditions, there was some reduction in the size of the tortuous seminal vesicles compared with control animals. Microscopically, it was found that most of the tortuous seminal vesicles are in a state of devastation and degeneration of spermatogenic epithelial cells. Foci with dystrophically altered and necrotized spermatogenic epithelium were also common. Arteries and veins of the testis were also subjected to simulated structural pathology. The wall of the arteries is thickened, their lumen is narrowed, some endothelial cells are dystrophically and necrobiotically altered and desquamated. In the venules and veins of the testis in the conditions of this experiment were observed plethora, blood stasis, stagnation. The contours of the walls of venous vessels are blurred, thickened. Swollen and sclerosed foci alternate, their contours are tortuous, twisted, and the lumens are deformed. Endothelial cells of venous vessels are enlarged in size with the phenomena of edema, dystrophy, necrobiosis. There were foci of endothelial cell desquamation, pyknosis and lysis of their nuclei. Prolonged exposure of experimental animals to rubomycin hydrochloride leads to a pronounced structural rearrangement of the arterial and venous channels, parenchyma and testicular stroma, which can significantly impair its spermatogenic and endocrine function.

Keywords

• rubomycin hydrochloride
• testis
• structures.