The development and application of a novel reagent for fixing red blood cells with glutaraldehyde and paraformaldehyde
Xinyang Li,
Miyang Li,
Yuhong Wang,
Shengbao Duan,
Hongmei Wang,
Yong Li,
Zhonghe Cai,
Ruiyao Wang,
Shuang Gao,
Yan Qu,
Tianxia Wang,
Fei Cheng,
Tiemei Liu
Affiliations
Xinyang Li
Department of Blood Transfusion, China–Japan Union Hospital of Jilin University, Changchun, People’s Republic of China
Miyang Li
Department of Laboratory, China–Japan Union Hospital of Jilin University, Changchun, People’s Republic of China
Yuhong Wang
Department of Blood Transfusion, Population life science and technology research institute in Jilin province, Changchun, People’s Republic of China
Shengbao Duan
CAS Key Lab of Bio-Medical Diagnostics, Suzhou Institute of Biomedical Engineering and Technology, Chinese Academy of Sciences, Suzhou, People’s Republic of China
Hongmei Wang
CAS Key Lab of Bio-Medical Diagnostics, Suzhou Institute of Biomedical Engineering and Technology, Chinese Academy of Sciences, Suzhou, People’s Republic of China
Yong Li
CAS Key Lab of Bio-Medical Diagnostics, Suzhou Institute of Biomedical Engineering and Technology, Chinese Academy of Sciences, Suzhou, People’s Republic of China
Zhonghe Cai
Department of Blood Transfusion, Population life science and technology research institute in Jilin province, Changchun, People’s Republic of China
Ruiyao Wang
Department of Blood Transfusion, Population life science and technology research institute in Jilin province, Changchun, People’s Republic of China
Shuang Gao
Department of Blood Transfusion, Population life science and technology research institute in Jilin province, Changchun, People’s Republic of China
Yan Qu
Department of Blood Transfusion, Population life science and technology research institute in Jilin province, Changchun, People’s Republic of China
Tianxia Wang
Department of Blood Transfusion, Population life science and technology research institute in Jilin province, Changchun, People’s Republic of China
Fei Cheng
Department of Laboratory,Population life science and technology research institute in Jilin province, Changchun, People’s Republic of China
Tiemei Liu
Department of Blood Transfusion, China–Japan Union Hospital of Jilin University, Changchun, People’s Republic of China
ABSTRACTObjective The currently employed red blood cell reagents have a short shelf life. Some hospitals with a small number of specimens will be unable to utilize them within the validity period, resulting in a substantial increase in the purchase price. Therefore, the method of developing long-term red blood cell reagents is a problem worthy of further study.Methods In this experiment, the type and concentration of the red blood cell reagent treatment solution were evaluated based on the red blood cell antigen concentration 24 h after treatment. In addition, the qualified glutaraldehyde/paraformaldehyde reagent was stored for six months, and five red blood cell indices were measured every month. At the same time, the detection indices of treated red blood cell reagents and untreated red blood cell reagents were compared.Results It was discovered that treated red blood cells containing 0.005% GA and 0.05% PFA were more suitable for the preservation of red blood cells than other treated concentrations, and the preservation time could reach six months. The test tube method (n = 24) and microcolumn gel card (n = 35) were used to determine the accuracy of the treated blood cells containing 0.005% glutaraldehyde +0.05% paraformaldehyde, with an accuracy of 100%.Conclusion This experiment resulted in the development of a novel reagent for treating red blood cells with glutaraldehyde/paraformaldehyde fixed solution that can effectively prolong its storage time by two to three times that of red blood cell reagents currently on the market.
