Communications Biology (Dec 2021)

PUFA synthase-independent DHA synthesis pathway in Parietichytrium sp. and its modification to produce EPA and n-3DPA

  • Yohei Ishibashi,
  • Hatsumi Goda,
  • Rie Hamaguchi,
  • Keishi Sakaguchi,
  • Takayoshi Sekiguchi,
  • Yuko Ishiwata,
  • Yuji Okita,
  • Seiya Mochinaga,
  • Shingo Ikeuchi,
  • Takahiro Mizobuchi,
  • Yoshitake Takao,
  • Kazuki Mori,
  • Kosuke Tashiro,
  • Nozomu Okino,
  • Daiske Honda,
  • Masahiro Hayashi,
  • Makoto Ito

DOI
https://doi.org/10.1038/s42003-021-02857-w
Journal volume & issue
Vol. 4, no. 1
pp. 1 – 11

Abstract

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Yohei Ishibashi and co-workers report that Parietichytrium sp. synthesizes DHA only via the elongase/desaturase pathway. They then engineered this thraustochytrid to produce EPA and n-3DPA by disrupting C20ELO and delta4 DES genes, respectively, in this biosynthetic pathway, suggesting that this may be a suitable system for the large scale production of these omega-3 fatty acids.