Identification and characterization of high-mobility group box 1 (HMGB1) from Nile tilapia (Oreochromis niloticus)

Jinzhong Niu; Fenglei Wu; Yu Huang; Jufen Tang; Bei Wang; Yishan Lu; Jia Cai; Jichang Jian

Aquaculture Reports (Mar 2021)

Identification and characterization of high-mobility group box 1 (HMGB1) from Nile tilapia (Oreochromis niloticus)

Jinzhong Niu,
Fenglei Wu,
Yu Huang,
Jufen Tang,
Bei Wang,
Yishan Lu,
Jia Cai,
Jichang Jian

Affiliations

Jinzhong Niu: College of Fishery, Guangdong Ocean University, Guangdong Provincial Key Laboratory of Pathogenic Biology and Epidemiology for Aquatic Economic Animal, Key Laboratory of Control for Disease of Aquatic Animals of Guangdong Higher Education Institutes, Southern Marine Science and Engineering Guangdong Laboratory, Zhanjiang, GD, China
Fenglei Wu: College of Fishery, Guangdong Ocean University, Guangdong Provincial Key Laboratory of Pathogenic Biology and Epidemiology for Aquatic Economic Animal, Key Laboratory of Control for Disease of Aquatic Animals of Guangdong Higher Education Institutes, Southern Marine Science and Engineering Guangdong Laboratory, Zhanjiang, GD, China
Yu Huang: College of Fishery, Guangdong Ocean University, Guangdong Provincial Key Laboratory of Pathogenic Biology and Epidemiology for Aquatic Economic Animal, Key Laboratory of Control for Disease of Aquatic Animals of Guangdong Higher Education Institutes, Southern Marine Science and Engineering Guangdong Laboratory, Zhanjiang, GD, China; Laboratory for MarineBiology and Biotechnology, Qingdao National Laboratory for Marine Science and Technology, Qingdao, China; Guangdong Provincial Engineering Research Center for Aquatic Animal Health Assessment, Shenzhen, 518120, China
Jufen Tang: College of Fishery, Guangdong Ocean University, Guangdong Provincial Key Laboratory of Pathogenic Biology and Epidemiology for Aquatic Economic Animal, Key Laboratory of Control for Disease of Aquatic Animals of Guangdong Higher Education Institutes, Southern Marine Science and Engineering Guangdong Laboratory, Zhanjiang, GD, China; Laboratory for MarineBiology and Biotechnology, Qingdao National Laboratory for Marine Science and Technology, Qingdao, China; Guangdong Provincial Engineering Research Center for Aquatic Animal Health Assessment, Shenzhen, 518120, China
Bei Wang: College of Fishery, Guangdong Ocean University, Guangdong Provincial Key Laboratory of Pathogenic Biology and Epidemiology for Aquatic Economic Animal, Key Laboratory of Control for Disease of Aquatic Animals of Guangdong Higher Education Institutes, Southern Marine Science and Engineering Guangdong Laboratory, Zhanjiang, GD, China; Laboratory for MarineBiology and Biotechnology, Qingdao National Laboratory for Marine Science and Technology, Qingdao, China; Guangdong Provincial Engineering Research Center for Aquatic Animal Health Assessment, Shenzhen, 518120, China
Yishan Lu: College of Fishery, Guangdong Ocean University, Guangdong Provincial Key Laboratory of Pathogenic Biology and Epidemiology for Aquatic Economic Animal, Key Laboratory of Control for Disease of Aquatic Animals of Guangdong Higher Education Institutes, Southern Marine Science and Engineering Guangdong Laboratory, Zhanjiang, GD, China; Laboratory for MarineBiology and Biotechnology, Qingdao National Laboratory for Marine Science and Technology, Qingdao, China; Guangdong Provincial Engineering Research Center for Aquatic Animal Health Assessment, Shenzhen, 518120, China
Jia Cai: College of Fishery, Guangdong Ocean University, Guangdong Provincial Key Laboratory of Pathogenic Biology and Epidemiology for Aquatic Economic Animal, Key Laboratory of Control for Disease of Aquatic Animals of Guangdong Higher Education Institutes, Southern Marine Science and Engineering Guangdong Laboratory, Zhanjiang, GD, China; Laboratory for MarineBiology and Biotechnology, Qingdao National Laboratory for Marine Science and Technology, Qingdao, China; Guangdong Provincial Engineering Research Center for Aquatic Animal Health Assessment, Shenzhen, 518120, China; Guangxi Key Lab for Marine Natural Products and Combinational Biosynthesis Chemistry, Guangxi Beibu Gulf Marine Research Centre, Guangxi Academy of Sciences, Nanning, China; Corresponding authors at: College of Fishery, Guangdong Ocean University, Guangdong Provincial Key Laboratory of Pathogenic Biology and Epidemiology for Aquatic Economic Animal, Key Laboratory of Control for Disease of Aquatic Animals of Guangdong Higher Education Institutes, Southern Marine Science and Engineering Guangdong Laboratory, Zhanjiang, GD, China.
Jichang Jian: College of Fishery, Guangdong Ocean University, Guangdong Provincial Key Laboratory of Pathogenic Biology and Epidemiology for Aquatic Economic Animal, Key Laboratory of Control for Disease of Aquatic Animals of Guangdong Higher Education Institutes, Southern Marine Science and Engineering Guangdong Laboratory, Zhanjiang, GD, China; Laboratory for MarineBiology and Biotechnology, Qingdao National Laboratory for Marine Science and Technology, Qingdao, China; Guangdong Provincial Engineering Research Center for Aquatic Animal Health Assessment, Shenzhen, 518120, China; Corresponding authors at: College of Fishery, Guangdong Ocean University, Guangdong Provincial Key Laboratory of Pathogenic Biology and Epidemiology for Aquatic Economic Animal, Key Laboratory of Control for Disease of Aquatic Animals of Guangdong Higher Education Institutes, Southern Marine Science and Engineering Guangdong Laboratory, Zhanjiang, GD, China.

Journal volume & issue: Vol. 19
p. 100573

Abstract

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High-mobility group box1 (HMGB1) is a non-histone chromosome protein that not only participates in nuclear formation and transcriptional regulation of, but also acts as a pro-inflammatory factor. In this study, a HMGB1 gene (OnHMGB1) was identified and characterized from Nile tilapia (Oreochromis niloticus). The open reading frame of OnHMGB1 sequence was 621 bp, encoding a 207 amino acids peptide. The deduced OnHMGB1 protein contained two HMG boxes and an acidic tail, which shared high identities to other reported HMGB1. OnHMGB1 was widely distributed in many tissues of healthy tilapia and was significantly up-regulated in spleen and head kidney after Streptococcus agalactiae poly I:C stimulation in vivo. Moreover, the recombinant protein of OnHMGB1 (rOnHMGB1) could bind to DNA in a dose-dependent manner, promote inflammatory factor expression and bactericidal activity of monocytes/macrophages in vitro as well. Taken together, these results indicate that OnHMGB1 plays roles in immune response of Nile tilapia against pathogen infection.

Published in Aquaculture Reports

ISSN: 2352-5134 (Online)
Publisher: Elsevier
Country of publisher: Netherlands
LCC subjects: Agriculture: Aquaculture. Fisheries. Angling
Website: http://www.journals.elsevier.com/aquaculture-reports/

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