A Study of Phytochemistry, Genoprotective Activity, and Antitumor Effects of Extracts of the Selected Lamiaceae Species
Mariana Oalđe Pavlović,
Stoimir Kolarević,
Jelena Đorđević,
Jovana Jovanović Marić,
Tanja Lunić,
Marija Mandić,
Margareta Kračun Kolarević,
Jelena Živković,
Ana Alimpić Aradski,
Petar D. Marin,
Katarina Šavikin,
Branka Vuković-Gačić,
Biljana Božić Nedeljković,
Sonja Duletić-Laušević
Affiliations
Mariana Oalđe Pavlović
Department of Plant Morphology and Systematics, Faculty of Biology, Institute of Botany and Botanical Garden “Jevremovac”, University of Belgrade, Studentski trg 16, 11070 Belgrade, Serbia
Stoimir Kolarević
Centre for Genotoxicology and Ecogenotoxicology, Department of Microbiology, Faculty of Biology, Institute of Botany and Botanical Garden “Jevremovac”, University of Belgrade, Studentski trg 16, 11070 Belgrade, Serbia
Jelena Đorđević
Centre for Genotoxicology and Ecogenotoxicology, Department of Microbiology, Faculty of Biology, Institute of Botany and Botanical Garden “Jevremovac”, University of Belgrade, Studentski trg 16, 11070 Belgrade, Serbia
Jovana Jovanović Marić
Centre for Genotoxicology and Ecogenotoxicology, Department of Microbiology, Faculty of Biology, Institute of Botany and Botanical Garden “Jevremovac”, University of Belgrade, Studentski trg 16, 11070 Belgrade, Serbia
Tanja Lunić
Faculty of Biology, Institute of Physiology and Biochemistry “Ivan Djaja”, University of Belgrade, Studentski trg 16, 11070 Belgrade, Serbia
Marija Mandić
Faculty of Biology, Institute of Physiology and Biochemistry “Ivan Djaja”, University of Belgrade, Studentski trg 16, 11070 Belgrade, Serbia
Margareta Kračun Kolarević
Department of Hydroecology and Water Protection, Institute for Biological Research “Siniša Stanković”, National Institute of Republic of Serbia, University of Belgrade, Bulevar Despota Stefana 142, 11070 Belgrade, Serbia
Jelena Živković
Institute for Medicinal Plants Research “Dr. Josif Pančić”, Tadeuša Košćuška 1,11070 Belgrade, Serbia
Ana Alimpić Aradski
Department of Plant Morphology and Systematics, Faculty of Biology, Institute of Botany and Botanical Garden “Jevremovac”, University of Belgrade, Studentski trg 16, 11070 Belgrade, Serbia
Petar D. Marin
Department of Plant Morphology and Systematics, Faculty of Biology, Institute of Botany and Botanical Garden “Jevremovac”, University of Belgrade, Studentski trg 16, 11070 Belgrade, Serbia
Katarina Šavikin
Institute for Medicinal Plants Research “Dr. Josif Pančić”, Tadeuša Košćuška 1,11070 Belgrade, Serbia
Branka Vuković-Gačić
Centre for Genotoxicology and Ecogenotoxicology, Department of Microbiology, Faculty of Biology, Institute of Botany and Botanical Garden “Jevremovac”, University of Belgrade, Studentski trg 16, 11070 Belgrade, Serbia
Biljana Božić Nedeljković
Faculty of Biology, Institute of Physiology and Biochemistry “Ivan Djaja”, University of Belgrade, Studentski trg 16, 11070 Belgrade, Serbia
Sonja Duletić-Laušević
Department of Plant Morphology and Systematics, Faculty of Biology, Institute of Botany and Botanical Garden “Jevremovac”, University of Belgrade, Studentski trg 16, 11070 Belgrade, Serbia
This study was designed to evaluate the genoprotective, antigenotoxic, as well as antitumor potential of methanolic, ethanolic, and aqueous extracts of Melissa officinalis, Mentha × piperita, Ocimum basilicum, Rosmarinus officinalis, Salvia officinalis, and Satureja montana (Lamiaceae), in different model systems. The polyphenols in these extracts were quantified both spectrophotometrically and using HPLC-DAD technique, while DPPH assay was used to assess the antioxidant activity. The genoprotective potential was tested on pUC19 Escherichia coli XL1-blue, and the antigenotoxicity on Salmonella typhimurium TA1535/pSK1002 and human lung fibroblasts, while the antitumor activity was assessed on colorectal cancer cells. Rosmarinic acid, quercetin, rutin, and luteolin-7-O-glucoside were among the identified compounds. Methanolic extracts had the best DPPH-scavenging and SOS-inducing activities, while ethanolic extracts exhibited the highest antigenotoxicity. Additionally, all extracts exhibited genoprotective potential on plasmid DNA. The antitumor effect was mediated by modulation of reactive oxygen species (ROS), nitric oxide (NO) production, and exhibition of genotoxic effects on tumor cells, especially with O. basilicum ethanolic extract. Generally, the investigated extracts were able to provide antioxidant protection for the acellular, prokaryotic, and normal human DNA, while also modulating the production of ROS and NO in tumor cells, leading to genotoxicity toward these cells and their decrease in proliferation.