Nature Communications (May 2020)
Ultrastructural visualization of 3D chromatin folding using volume electron microscopy and DNA in situ hybridization
- Paweł Trzaskoma,
- Błażej Ruszczycki,
- Byoungkoo Lee,
- Katarzyna K. Pels,
- Katarzyna Krawczyk,
- Grzegorz Bokota,
- Andrzej A. Szczepankiewicz,
- Jesse Aaron,
- Agnieszka Walczak,
- Małgorzata A. Śliwińska,
- Adriana Magalska,
- Michal Kadlof,
- Artur Wolny,
- Zofia Parteka,
- Sebastian Arabasz,
- Magdalena Kiss-Arabasz,
- Dariusz Plewczyński,
- Yijun Ruan,
- Grzegorz M. Wilczyński
Affiliations
- Paweł Trzaskoma
- Nencki Institute of Experimental Biology, Polish Academy of Sciences
- Błażej Ruszczycki
- Nencki Institute of Experimental Biology, Polish Academy of Sciences
- Byoungkoo Lee
- The Jackson Laboratory for Genomic Medicine, 10 Discovery Dr
- Katarzyna K. Pels
- Nencki Institute of Experimental Biology, Polish Academy of Sciences
- Katarzyna Krawczyk
- Nencki Institute of Experimental Biology, Polish Academy of Sciences
- Grzegorz Bokota
- Center of New Technologies, University of Warsaw
- Andrzej A. Szczepankiewicz
- Nencki Institute of Experimental Biology, Polish Academy of Sciences
- Jesse Aaron
- Janelia Research Campus, Howard Hughes Medical Institute
- Agnieszka Walczak
- Nencki Institute of Experimental Biology, Polish Academy of Sciences
- Małgorzata A. Śliwińska
- Nencki Institute of Experimental Biology, Polish Academy of Sciences
- Adriana Magalska
- Nencki Institute of Experimental Biology, Polish Academy of Sciences
- Michal Kadlof
- Center of New Technologies, University of Warsaw
- Artur Wolny
- Nencki Institute of Experimental Biology, Polish Academy of Sciences
- Zofia Parteka
- Center of New Technologies, University of Warsaw
- Sebastian Arabasz
- Łukasiewicz Research NETWORK – PORT Polish Center for Technology Development
- Magdalena Kiss-Arabasz
- Łukasiewicz Research NETWORK – PORT Polish Center for Technology Development
- Dariusz Plewczyński
- Center of New Technologies, University of Warsaw
- Yijun Ruan
- The Jackson Laboratory for Genomic Medicine, 10 Discovery Dr
- Grzegorz M. Wilczyński
- Nencki Institute of Experimental Biology, Polish Academy of Sciences
- DOI
- https://doi.org/10.1038/s41467-020-15987-2
- Journal volume & issue
-
Vol. 11,
no. 1
pp. 1 – 9
Abstract
The genome is folded in 3-dimensions, though the lack of robust ultra-resolution imaging makes this difficult to visualise. Here, the authors present 3D-EMISH that combines serial block-face scanning electron microscopy with in situ hybridization.
