PREVENTION OF BACTERIAL COMPLICATIONS CORRECTION APOPTOSIS OF T-LYMPHOCYTES IN NEWBORNS WITH RESPIRATORY DISEASE

Abstract

Purpose: prevention of bacterial complications correction apoptosis of T lymphocytes with human recombinant granulocyte colony-stimulating factor (G-CSF-par).Materials and Methods: In randomized, controlled, blind clinical study included 69 neonates with respiratory pathology on mechanical ventilation who were born at term 39,2 ± 1,1 weeks, with a mass of 3650 ± 421, Apgar score 2,3±1,1, no clinical signs of infection, containing lymphocytes in early (AnnexinV-FITC + PI-) and late (AnnexinV-FITC + PI +) apoptosis than 9.59% and 0.56% (respectively) distributed in groups: patients I ( n = 39) received G-CSF is a par-10 mg / kg / day intravenously for the first 3 days, in group II (n = 30) par-G-CSF was not given. On admission, for 3-5 days and in the outcome of the disease by ELISA studied the content of G-CSF, FGF, sFas-L; subpopulations of lymphocytes by flow cytometry on a flow cytometer, Beckman Coulter Epics XL (USA).Results: Statistical analysis of the results confirmed the reduction of the relative amount of lymphocytes activated Fas-receptor (CD95) and lymphocytes repositories early and late apoptosis, increasing stem cells in peripheral blood, elevated levels of FGF and antiapoptogennogo sFAS after administration par-GCSF, decrease the incidence of septic complications.Summary: Timely correction of apoptosis of T-lymphocytes par-G-CSF is effective and safe profilaktiruet onset sepsis in neonates with respiratory disorders who are on mechanical ventilation.

Keywords

• apoptosis
• cytokines
• sepsis
• newborn