Nature Communications (Sep 2021)
Confronting false discoveries in single-cell differential expression
- Jordan W. Squair,
- Matthieu Gautier,
- Claudia Kathe,
- Mark A. Anderson,
- Nicholas D. James,
- Thomas H. Hutson,
- Rémi Hudelle,
- Taha Qaiser,
- Kaya J. E. Matson,
- Quentin Barraud,
- Ariel J. Levine,
- Gioele La Manno,
- Michael A. Skinnider,
- Grégoire Courtine
Affiliations
- Jordan W. Squair
- Center for Neuroprosthetics and Brain Mind Institute, Faculty of Life Sciences, École Polytechnique Fédérale de Lausanne (EPFL)
- Matthieu Gautier
- Center for Neuroprosthetics and Brain Mind Institute, Faculty of Life Sciences, École Polytechnique Fédérale de Lausanne (EPFL)
- Claudia Kathe
- Center for Neuroprosthetics and Brain Mind Institute, Faculty of Life Sciences, École Polytechnique Fédérale de Lausanne (EPFL)
- Mark A. Anderson
- Center for Neuroprosthetics and Brain Mind Institute, Faculty of Life Sciences, École Polytechnique Fédérale de Lausanne (EPFL)
- Nicholas D. James
- Center for Neuroprosthetics and Brain Mind Institute, Faculty of Life Sciences, École Polytechnique Fédérale de Lausanne (EPFL)
- Thomas H. Hutson
- Center for Neuroprosthetics and Brain Mind Institute, Faculty of Life Sciences, École Polytechnique Fédérale de Lausanne (EPFL)
- Rémi Hudelle
- Center for Neuroprosthetics and Brain Mind Institute, Faculty of Life Sciences, École Polytechnique Fédérale de Lausanne (EPFL)
- Taha Qaiser
- International Collaboration on Repair Discoveries (ICORD), University of British Columbia
- Kaya J. E. Matson
- Spinal Circuits and Plasticity Unit, National Institute of Neurological Disorders and Stroke
- Quentin Barraud
- Center for Neuroprosthetics and Brain Mind Institute, Faculty of Life Sciences, École Polytechnique Fédérale de Lausanne (EPFL)
- Ariel J. Levine
- Spinal Circuits and Plasticity Unit, National Institute of Neurological Disorders and Stroke
- Gioele La Manno
- Center for Neuroprosthetics and Brain Mind Institute, Faculty of Life Sciences, École Polytechnique Fédérale de Lausanne (EPFL)
- Michael A. Skinnider
- Center for Neuroprosthetics and Brain Mind Institute, Faculty of Life Sciences, École Polytechnique Fédérale de Lausanne (EPFL)
- Grégoire Courtine
- Center for Neuroprosthetics and Brain Mind Institute, Faculty of Life Sciences, École Polytechnique Fédérale de Lausanne (EPFL)
- DOI
- https://doi.org/10.1038/s41467-021-25960-2
- Journal volume & issue
-
Vol. 12,
no. 1
pp. 1 – 15
Abstract
Differential expression analysis of single-cell transcriptomics allows scientists to dissect cell-type-specific responses to biological perturbations. Here, the authors show that many commonly used methods are biased and can produce false discoveries.
