Nature Communications (Apr 2021)
T cell assays differentiate clinical and subclinical SARS-CoV-2 infections from cross-reactive antiviral responses
- Ane Ogbe,
- Barbara Kronsteiner,
- Donal T. Skelly,
- Matthew Pace,
- Anthony Brown,
- Emily Adland,
- Kareena Adair,
- Hossain Delowar Akhter,
- Mohammad Ali,
- Serat-E Ali,
- Adrienn Angyal,
- M. Azim Ansari,
- Carolina V. Arancibia-Cárcamo,
- Helen Brown,
- Senthil Chinnakannan,
- Christopher Conlon,
- Catherine de Lara,
- Thushan de Silva,
- Christina Dold,
- Tao Dong,
- Timothy Donnison,
- David Eyre,
- Amy Flaxman,
- Helen Fletcher,
- Joshua Gardner,
- James T. Grist,
- Carl-Philipp Hackstein,
- Kanoot Jaruthamsophon,
- Katie Jeffery,
- Teresa Lambe,
- Lian Lee,
- Wenqin Li,
- Nicholas Lim,
- Philippa C. Matthews,
- Alexander J. Mentzer,
- Shona C. Moore,
- Dean J. Naisbitt,
- Monday Ogese,
- Graham Ogg,
- Peter Openshaw,
- Munir Pirmohamed,
- Andrew J. Pollard,
- Narayan Ramamurthy,
- Patpong Rongkard,
- Sarah Rowland-Jones,
- Oliver Sampson,
- Gavin Screaton,
- Alessandro Sette,
- Lizzie Stafford,
- Craig Thompson,
- Paul J. Thomson,
- Ryan Thwaites,
- Vinicius Vieira,
- Daniela Weiskopf,
- Panagiota Zacharopoulou,
- Oxford Immunology Network Covid-19 Response T Cell Consortium,
- Oxford Protective T Cell Immunology for COVID-19 (OPTIC) Clinical Team,
- Lance Turtle,
- Paul Klenerman,
- Philip Goulder,
- John Frater,
- Eleanor Barnes,
- Susanna Dunachie
Affiliations
- Ane Ogbe
- Nuffield Department of Clinical Medicine, Peter Medawar Building for Pathogen Research, University of Oxford
- Barbara Kronsteiner
- Nuffield Department of Clinical Medicine, Peter Medawar Building for Pathogen Research, University of Oxford
- Donal T. Skelly
- Nuffield Department of Clinical Medicine, Peter Medawar Building for Pathogen Research, University of Oxford
- Matthew Pace
- Nuffield Department of Clinical Medicine, Peter Medawar Building for Pathogen Research, University of Oxford
- Anthony Brown
- Nuffield Department of Clinical Medicine, Peter Medawar Building for Pathogen Research, University of Oxford
- Emily Adland
- Nuffield Department of Clinical Medicine, Peter Medawar Building for Pathogen Research, University of Oxford
- Kareena Adair
- Department of Molecular and Clinical Pharmacology, MRC Centre for Drug Safety Science, University of Liverpool
- Hossain Delowar Akhter
- Nuffield Department of Clinical Medicine, Peter Medawar Building for Pathogen Research, University of Oxford
- Mohammad Ali
- Nuffield Department of Clinical Medicine, Peter Medawar Building for Pathogen Research, University of Oxford
- Serat-E Ali
- Department of Molecular and Clinical Pharmacology, MRC Centre for Drug Safety Science, University of Liverpool
- Adrienn Angyal
- The Florey Institute for Host-Pathogen Interactions and Department of Infection, Immunity and Cardiovascular Disease, Medical School, University of Sheffield
- M. Azim Ansari
- Nuffield Department of Clinical Medicine, Peter Medawar Building for Pathogen Research, University of Oxford
- Carolina V. Arancibia-Cárcamo
- Nuffield Department of Clinical Neurosciences, University of Oxford
- Helen Brown
- Nuffield Department of Clinical Medicine, Peter Medawar Building for Pathogen Research, University of Oxford
- Senthil Chinnakannan
- Nuffield Department of Clinical Medicine, Peter Medawar Building for Pathogen Research, University of Oxford
- Christopher Conlon
- Nuffield Department of Clinical Medicine, Centre for Tropical Medicine and Global Health, University of Oxford
- Catherine de Lara
- Nuffield Department of Clinical Medicine, Peter Medawar Building for Pathogen Research, University of Oxford
- Thushan de Silva
- The Florey Institute for Host-Pathogen Interactions and Department of Infection, Immunity and Cardiovascular Disease, Medical School, University of Sheffield
- Christina Dold
- Oxford Vaccine Group, Department of Paediatrics, University of Oxford
- Tao Dong
- MRC Human Immunology Unit, MRC Weatherall Institute of Molecular Medicine, University of Oxford
- Timothy Donnison
- Nuffield Department of Clinical Medicine, Peter Medawar Building for Pathogen Research, University of Oxford
- David Eyre
- Oxford University Hospitals NHS Foundation Trust
- Amy Flaxman
- Jenner Institute, University of Oxford
- Helen Fletcher
- Department of Infection Biology, Faculty of Infectious and Tropical Diseases, London School of Hygiene and Tropical Medicine
- Joshua Gardner
- Department of Molecular and Clinical Pharmacology, MRC Centre for Drug Safety Science, University of Liverpool
- James T. Grist
- NIHR Oxford Biomedical Research Centre, University of Oxford
- Carl-Philipp Hackstein
- Nuffield Department of Clinical Medicine, Peter Medawar Building for Pathogen Research, University of Oxford
- Kanoot Jaruthamsophon
- Department of Molecular and Clinical Pharmacology, MRC Centre for Drug Safety Science, University of Liverpool
- Katie Jeffery
- Oxford University Hospitals NHS Foundation Trust
- Teresa Lambe
- Jenner Institute, University of Oxford
- Lian Lee
- Nuffield Department of Clinical Medicine, Peter Medawar Building for Pathogen Research, University of Oxford
- Wenqin Li
- Nuffield Department of Clinical Medicine, Peter Medawar Building for Pathogen Research, University of Oxford
- Nicholas Lim
- Nuffield Department of Clinical Medicine, Peter Medawar Building for Pathogen Research, University of Oxford
- Philippa C. Matthews
- Nuffield Department of Clinical Medicine, Peter Medawar Building for Pathogen Research, University of Oxford
- Alexander J. Mentzer
- Oxford University Hospitals NHS Foundation Trust
- Shona C. Moore
- HPRU in Emerging and Zoonotic Infections, Institute of Infection, Veterinary and Ecological Sciences, University of Liverpool
- Dean J. Naisbitt
- Department of Molecular and Clinical Pharmacology, MRC Centre for Drug Safety Science, University of Liverpool
- Monday Ogese
- Department of Molecular and Clinical Pharmacology, MRC Centre for Drug Safety Science, University of Liverpool
- Graham Ogg
- Oxford University Hospitals NHS Foundation Trust
- Peter Openshaw
- Faculty of Medicine, National Heart and Lung institute, Imperial College
- Munir Pirmohamed
- Department of Molecular and Clinical Pharmacology, MRC Centre for Drug Safety Science, University of Liverpool
- Andrew J. Pollard
- Oxford Vaccine Group, Department of Paediatrics, University of Oxford
- Narayan Ramamurthy
- Nuffield Department of Clinical Medicine, Peter Medawar Building for Pathogen Research, University of Oxford
- Patpong Rongkard
- Nuffield Department of Clinical Medicine, Peter Medawar Building for Pathogen Research, University of Oxford
- Sarah Rowland-Jones
- The Florey Institute for Host-Pathogen Interactions and Department of Infection, Immunity and Cardiovascular Disease, Medical School, University of Sheffield
- Oliver Sampson
- Nuffield Department of Clinical Medicine, Peter Medawar Building for Pathogen Research, University of Oxford
- Gavin Screaton
- Wellcome Centre for Human Genetics, University of Oxford
- Alessandro Sette
- Center for Infectious Disease and Vaccine Research, La Jolla Institute for Immunology
- Lizzie Stafford
- Oxford University Hospitals NHS Foundation Trust
- Craig Thompson
- Peter Medawar Building for Pathogen Research, Department of Zoology, University of Oxford
- Paul J. Thomson
- Department of Molecular and Clinical Pharmacology, MRC Centre for Drug Safety Science, University of Liverpool
- Ryan Thwaites
- Faculty of Medicine, National Heart and Lung institute, Imperial College
- Vinicius Vieira
- Nuffield Department of Clinical Medicine, Peter Medawar Building for Pathogen Research, University of Oxford
- Daniela Weiskopf
- Center for Infectious Disease and Vaccine Research, La Jolla Institute for Immunology
- Panagiota Zacharopoulou
- Nuffield Department of Clinical Medicine, Peter Medawar Building for Pathogen Research, University of Oxford
- Oxford Immunology Network Covid-19 Response T Cell Consortium
- Oxford Protective T Cell Immunology for COVID-19 (OPTIC) Clinical Team
- Lance Turtle
- HPRU in Emerging and Zoonotic Infections, Institute of Infection, Veterinary and Ecological Sciences, University of Liverpool
- Paul Klenerman
- Nuffield Department of Clinical Medicine, Peter Medawar Building for Pathogen Research, University of Oxford
- Philip Goulder
- Peter Medawar Building for Pathogen Research, Department of Paediatrics, University of Oxford
- John Frater
- Nuffield Department of Clinical Medicine, Peter Medawar Building for Pathogen Research, University of Oxford
- Eleanor Barnes
- Nuffield Department of Clinical Medicine, Peter Medawar Building for Pathogen Research, University of Oxford
- Susanna Dunachie
- Nuffield Department of Clinical Medicine, Peter Medawar Building for Pathogen Research, University of Oxford
- DOI
- https://doi.org/10.1038/s41467-021-21856-3
- Journal volume & issue
-
Vol. 12,
no. 1
pp. 1 – 14
Abstract
Understanding the immune response to SARS-CoV-2 is dependent on being able to distinguish COVID-19 immune responses from cross-reactive immune responses to other coronaviruses. Here the authors show that choice of antigens and whether an ICS, ELISPOT or T cell proliferation assay is used has a major effect on this discriminatory ability.
