Metabolic analysis of mouse bone-marrow-derived dendritic cells using an extracellular flux analyzer

Kazuhito Gotoh; Yurie Takata; Yuya Nakashima; Soichi Mizuguchi; Keishi Komori; Dongchon Kang

STAR Protocols (Jun 2021)

Metabolic analysis of mouse bone-marrow-derived dendritic cells using an extracellular flux analyzer

Kazuhito Gotoh,
Yurie Takata,
Yuya Nakashima,
Soichi Mizuguchi,
Keishi Komori,
Dongchon Kang

Affiliations

Kazuhito Gotoh: Department of Clinical Chemistry and Laboratory Medicine, Graduate School of Medical Sciences, Kyushu University, 3-1-1, Maidashi, Higashi-ku, Fukuoka 812-8582, Japan; Corresponding author
Yurie Takata: Department of Clinical Chemistry and Laboratory Medicine, Graduate School of Medical Sciences, Kyushu University, 3-1-1, Maidashi, Higashi-ku, Fukuoka 812-8582, Japan
Yuya Nakashima: Department of Clinical Chemistry and Laboratory Medicine, Graduate School of Medical Sciences, Kyushu University, 3-1-1, Maidashi, Higashi-ku, Fukuoka 812-8582, Japan
Soichi Mizuguchi: Department of Clinical Chemistry and Laboratory Medicine, Graduate School of Medical Sciences, Kyushu University, 3-1-1, Maidashi, Higashi-ku, Fukuoka 812-8582, Japan
Keishi Komori: Department of Clinical Chemistry and Laboratory Medicine, Graduate School of Medical Sciences, Kyushu University, 3-1-1, Maidashi, Higashi-ku, Fukuoka 812-8582, Japan
Dongchon Kang: Department of Clinical Chemistry and Laboratory Medicine, Graduate School of Medical Sciences, Kyushu University, 3-1-1, Maidashi, Higashi-ku, Fukuoka 812-8582, Japan; Corresponding author

Journal volume & issue: Vol. 2, no. 2
p. 100401

Abstract

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Summary: Dendritic cell (DC) maturation induced by Toll-like receptor (TLR) agonists requires the activation of downstream metabolic changes. Here, we provide a detailed protocol to measure glycolysis, mitochondrial respiration, and fatty acid oxidation in mouse bone-marrow-derived DCs with the Seahorse XF24 extracellular flux (XF) analyzer. XF analysis with the Seahorse bioanalyzer has become a standard method to measure bioenergetic functions in cells, and this protocol can be adapted to other immune cells.For complete information on using this protocol, please refer to Gotoh et al. (2018).

Published in STAR Protocols

ISSN: 2666-1667 (Online)
Publisher: Elsevier
Country of publisher: United States
LCC subjects: Science: Science (General)
Website: https://star-protocols.cell.com/

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