Nature Communications (Oct 2020)
Exploiting oxidative phosphorylation to promote the stem and immunoevasive properties of pancreatic cancer stem cells
- Sandra Valle,
- Sonia Alcalá,
- Laura Martin-Hijano,
- Pablo Cabezas-Sáinz,
- Diego Navarro,
- Edurne Ramos Muñoz,
- Lourdes Yuste,
- Kanishka Tiwary,
- Karolin Walter,
- Laura Ruiz-Cañas,
- Marta Alonso-Nocelo,
- Juan A. Rubiolo,
- Emilio González-Arnay,
- Christopher Heeschen,
- Laura Garcia-Bermejo,
- Patrick C. Hermann,
- Laura Sánchez,
- Patricia Sancho,
- Miguel Ángel Fernández-Moreno,
- Bruno Sainz
Affiliations
- Sandra Valle
- Department of Biochemistry, Universidad Autónoma de Madrid (UAM) and Instituto de Investigaciones Biomédicas “Alberto Sols” (IIBM), CSIC-UAM
- Sonia Alcalá
- Department of Biochemistry, Universidad Autónoma de Madrid (UAM) and Instituto de Investigaciones Biomédicas “Alberto Sols” (IIBM), CSIC-UAM
- Laura Martin-Hijano
- Department of Biochemistry, Universidad Autónoma de Madrid (UAM) and Instituto de Investigaciones Biomédicas “Alberto Sols” (IIBM), CSIC-UAM
- Pablo Cabezas-Sáinz
- Department of Zoology, Genetics and Physical Anthropology, Veterinary Faculty, Universidad de Santiago de Compostela
- Diego Navarro
- Department of Biochemistry, Universidad Autónoma de Madrid (UAM) and Instituto de Investigaciones Biomédicas “Alberto Sols” (IIBM), CSIC-UAM
- Edurne Ramos Muñoz
- Biomarkers and Therapeutic Targets Group - IRYCIS
- Lourdes Yuste
- Department of Biochemistry, Universidad Autónoma de Madrid (UAM) and Instituto de Investigaciones Biomédicas “Alberto Sols” (IIBM), CSIC-UAM
- Kanishka Tiwary
- Department of Internal Medicine I, Ulm University
- Karolin Walter
- Department of Internal Medicine I, Ulm University
- Laura Ruiz-Cañas
- Department of Biochemistry, Universidad Autónoma de Madrid (UAM) and Instituto de Investigaciones Biomédicas “Alberto Sols” (IIBM), CSIC-UAM
- Marta Alonso-Nocelo
- Department of Biochemistry, Universidad Autónoma de Madrid (UAM) and Instituto de Investigaciones Biomédicas “Alberto Sols” (IIBM), CSIC-UAM
- Juan A. Rubiolo
- Department of Zoology, Genetics and Physical Anthropology, Veterinary Faculty, Universidad de Santiago de Compostela
- Emilio González-Arnay
- Department of Neurology, Universidad Autónoma de Madrid (UAM)
- Christopher Heeschen
- Stem Cells & Cancer Group, Molecular Pathology Programme, Spanish National Cancer Research Centre (CNIO)
- Laura Garcia-Bermejo
- Biomarkers and Therapeutic Targets Group - IRYCIS
- Patrick C. Hermann
- Department of Internal Medicine I, Ulm University
- Laura Sánchez
- Department of Zoology, Genetics and Physical Anthropology, Veterinary Faculty, Universidad de Santiago de Compostela
- Patricia Sancho
- Translational Research Unit, IIS Aragón
- Miguel Ángel Fernández-Moreno
- Department of Biochemistry, Universidad Autónoma de Madrid (UAM) and Instituto de Investigaciones Biomédicas “Alberto Sols” (IIBM), CSIC-UAM
- Bruno Sainz
- Department of Biochemistry, Universidad Autónoma de Madrid (UAM) and Instituto de Investigaciones Biomédicas “Alberto Sols” (IIBM), CSIC-UAM
- DOI
- https://doi.org/10.1038/s41467-020-18954-z
- Journal volume & issue
-
Vol. 11,
no. 1
pp. 1 – 19
Abstract
Long-term cultures of pancreatic cancer stem cells (PaCSCs) are difficult to obtain. Here, the authors present a 2D culture method, based on the use of galactose, to establish cell cultures from pancreatic ductal adenocarcinoma xenotransplants enriched in PaCSCs.
