eLife (Apr 2017)
Oriented clonal cell dynamics enables accurate growth and shaping of vertebrate cartilage
- Marketa Kaucka,
- Tomas Zikmund,
- Marketa Tesarova,
- Daniel Gyllborg,
- Andreas Hellander,
- Josef Jaros,
- Jozef Kaiser,
- Julian Petersen,
- Bara Szarowska,
- Phillip T Newton,
- Vyacheslav Dyachuk,
- Lei Li,
- Hong Qian,
- Anne-Sofie Johansson,
- Yuji Mishina,
- Joshua D Currie,
- Elly M Tanaka,
- Alek Erickson,
- Andrew Dudley,
- Hjalmar Brismar,
- Paul Southam,
- Enrico Coen,
- Min Chen,
- Lee S Weinstein,
- Ales Hampl,
- Ernest Arenas,
- Andrei S Chagin,
- Kaj Fried,
- Igor Adameyko
Affiliations
- Marketa Kaucka
- ORCiD
- Department of Physiology and Pharmacology, Karolinska Institutet, Stockholm, Sweden; Center for Brain Research, Medical University Vienna, Vienna, Austria
- Tomas Zikmund
- Central European Institute of Technology, Brno University of Technology, Brno, Czech Republic
- Marketa Tesarova
- Central European Institute of Technology, Brno University of Technology, Brno, Czech Republic
- Daniel Gyllborg
- Unit of Molecular Neurobiology, Department of Medical Biochemistry and Biophysics, Karolinska Institutet, Stockholm, Sweden
- Andreas Hellander
- Department of Information Technology, Uppsala University, Uppsala, Sweden
- Josef Jaros
- Department of Histology and Embryology, Medical Faculty, Masaryk University, Brno, Czech Republic
- Jozef Kaiser
- Central European Institute of Technology, Brno University of Technology, Brno, Czech Republic
- Julian Petersen
- Center for Brain Research, Medical University Vienna, Vienna, Austria
- Bara Szarowska
- Center for Brain Research, Medical University Vienna, Vienna, Austria
- Phillip T Newton
- Department of Physiology and Pharmacology, Karolinska Institutet, Stockholm, Sweden
- Vyacheslav Dyachuk
- Department of Neuroscience, Karolinska Institutet, Stockholm, Sweden
- Lei Li
- Department of Physiology and Pharmacology, Karolinska Institutet, Stockholm, Sweden
- Hong Qian
- Department of Medicine, Karolinska Institutet, Stockholm, Sweden
- Anne-Sofie Johansson
- Department of Medicine, Karolinska Institutet, Stockholm, Sweden
- Yuji Mishina
- Department of Biologic and Materials Sciences, University of Michigan School of Dentistry, Ann Arbor, United States
- Joshua D Currie
- Center for Regenerative Therapies, Technische Universität Dresden, Dresden, Germany
- Elly M Tanaka
- Center for Regenerative Therapies, Technische Universität Dresden, Dresden, Germany
- Alek Erickson
- Department of Genetics, Cell Biology and Anatomy, University of Nebraska Medical Center, Omaha, United States
- Andrew Dudley
- Department of Genetics, Cell Biology and Anatomy, University of Nebraska Medical Center, Omaha, United States
- Hjalmar Brismar
- ORCiD
- Science for Life Laboratory, Royal Institute of Technology, Solna, Sweden
- Paul Southam
- John Innes Centre, Norwich, United Kingdom
- Enrico Coen
- ORCiD
- John Innes Centre, Norwich, United Kingdom
- Min Chen
- National Institute of Diabetes and Digestive and Kidney Diseases, National Institutes of Health, Bethesda, United States
- Lee S Weinstein
- National Institute of Diabetes and Digestive and Kidney Diseases, National Institutes of Health, Bethesda, United States
- Ales Hampl
- Department of Histology and Embryology, Medical Faculty, Masaryk University, Brno, Czech Republic
- Ernest Arenas
- Unit of Molecular Neurobiology, Department of Medical Biochemistry and Biophysics, Karolinska Institutet, Stockholm, Sweden
- Andrei S Chagin
- ORCiD
- Department of Physiology and Pharmacology, Karolinska Institutet, Stockholm, Sweden; Institute for Regenerative Medicine, Sechenov First Moscow State Medical University, Moscow, Russia
- Kaj Fried
- Department of Neuroscience, Karolinska Institutet, Stockholm, Sweden
- Igor Adameyko
- ORCiD
- Department of Physiology and Pharmacology, Karolinska Institutet, Stockholm, Sweden; Center for Brain Research, Medical University Vienna, Vienna, Austria
- DOI
- https://doi.org/10.7554/eLife.25902
- Journal volume & issue
-
Vol. 6
Abstract
Cartilaginous structures are at the core of embryo growth and shaping before the bone forms. Here we report a novel principle of vertebrate cartilage growth that is based on introducing transversally-oriented clones into pre-existing cartilage. This mechanism of growth uncouples the lateral expansion of curved cartilaginous sheets from the control of cartilage thickness, a process which might be the evolutionary mechanism underlying adaptations of facial shape. In rod-shaped cartilage structures (Meckel, ribs and skeletal elements in developing limbs), the transverse integration of clonal columns determines the well-defined diameter and resulting rod-like morphology. We were able to alter cartilage shape by experimentally manipulating clonal geometries. Using in silico modeling, we discovered that anisotropic proliferation might explain cartilage bending and groove formation at the macro-scale.
Keywords
- chondrocranium
- scaling and shaping
- facial cartilage growth
- mouse mutants
- mathematical and material modelling
- Wnt/PCP
