Differential adaptive responses to 1‐ or 2‐day fasting in various mouse tissues revealed by quantitative PCR analysis

Abstract

Dietary or caloric restriction confers various clinical benefits. Short‐term fasting of mice is a common experimental procedure that may involve systemic metabolic remodeling, which may significantly affect experimental outputs. This study evaluated adaptive cellular responses after 1‐ or 2‐day fasting in 13 mouse tissues by quantitative PCR using 15 marker primer sets for the activation of ubiquitin–proteasome (Atrogin‐1 andMuRF1), autophagy–lysosome (LC3b,p62 andLamp2), amino acid response (Asns,Trib3,Herpud1,xCT, andChop), Nrf2‐mediated antioxidant (HO‐1 andGsta1), and amino acid transport (Slc38a2,Slc7a5, andSlc7a1) systems. Differential activation profiles obtained in seven highly (thymus, liver, spleen, and small intestine) or mildly (stomach, kidney, and colon) atrophied tissues as well as in six non‐atrophied tissues (brain, eye, lung, heart, skeletal muscle, and testis) suggested tissue‐specific active metabolic remodeling.

Keywords

• Amino acid response
• Amino acid transport
• Autophagy
• Nrf2
• Proteasome
• Ubiquitin