Frontiers in Veterinary Science (Oct 2020)

Field-Adapted Full Genome Sequencing of Peste-Des-Petits-Ruminants Virus Using Nanopore Sequencing

  • Emeli Torsson,
  • Tebogo Kgotlele,
  • Gerald Misinzo,
  • Jonas Johansson Wensman,
  • Mikael Berg,
  • Oskar Karlsson Lindsjö

DOI
https://doi.org/10.3389/fvets.2020.542724
Journal volume & issue
Vol. 7

Abstract

Read online

Peste-des-petits-ruminants virus (PPRV) is currently the focus of a control and eradication program. Full genome sequencing has the opportunity to become a powerful tool in the eradication program by improving molecular epidemiology and the study of viral evolution. PPRV is prevalent in many resource-constrained areas, with long distances to laboratory facilities, which can lack the correct equipment for high-throughput sequencing. Here we present a protocol for near full or full genome sequencing of PPRV. The use of a portable miniPCR and MinION brings the laboratory to the field and in addition makes the production of a full genome possible within 24 h of sampling. The protocol has been successfully used on virus isolates from cell cultures and field isolates from tissue samples of naturally infected goats.

Keywords