Enhancing Resistance to Cercospora Leaf Spot in Mung Bean <i>(Vigna radiata</i> L.) through <i>Bradyrhizobium</i> sp. DOA9 Priming: Molecular Insights and Bio-Priming Potential
Apisit Songsaeng,
Pakpoom Boonchuen,
Phongkeat Nareephot,
Pongdet Piromyou,
Jenjira Wongdee,
Teerana Greetatorn,
Sukanya Inthaisong,
Piyada Alisha Tantasawat,
Kamonluck Teamtisong,
Panlada Tittabutr,
Shusei Sato,
Nantakorn Boonkerd,
Pongpan Songwattana,
Neung Teaumroong
Affiliations
Apisit Songsaeng
School of Biotechnology, Institute of Agricultural Technology, Suranaree University of Technology, Nakhon Ratchasima 30000, Thailand
Pakpoom Boonchuen
School of Biotechnology, Institute of Agricultural Technology, Suranaree University of Technology, Nakhon Ratchasima 30000, Thailand
Phongkeat Nareephot
School of Biotechnology, Institute of Agricultural Technology, Suranaree University of Technology, Nakhon Ratchasima 30000, Thailand
Pongdet Piromyou
Institute of Research and Development, Suranaree University of Technology, Nakhon Ratchasima 30000, Thailand
Jenjira Wongdee
Institute of Research and Development, Suranaree University of Technology, Nakhon Ratchasima 30000, Thailand
Teerana Greetatorn
Institute of Research and Development, Suranaree University of Technology, Nakhon Ratchasima 30000, Thailand
Sukanya Inthaisong
School of Crop Production Technology, Institute of Agricultural Technology, Suranaree University of Technology, Nakhon Ratchasima 30000, Thailand
Piyada Alisha Tantasawat
School of Crop Production Technology, Institute of Agricultural Technology, Suranaree University of Technology, Nakhon Ratchasima 30000, Thailand
Kamonluck Teamtisong
The Center for Scientific and Technological Equipment, Suranaree University of Technology, Nakhon Ratchasima 30000, Thailand
Panlada Tittabutr
School of Biotechnology, Institute of Agricultural Technology, Suranaree University of Technology, Nakhon Ratchasima 30000, Thailand
Shusei Sato
Graduate School of Life Sciences, Tohoku University, Sendai 980-8577, Japan
Nantakorn Boonkerd
School of Biotechnology, Institute of Agricultural Technology, Suranaree University of Technology, Nakhon Ratchasima 30000, Thailand
Pongpan Songwattana
Institute of Research and Development, Suranaree University of Technology, Nakhon Ratchasima 30000, Thailand
Neung Teaumroong
School of Biotechnology, Institute of Agricultural Technology, Suranaree University of Technology, Nakhon Ratchasima 30000, Thailand
Mung bean (Vigna radiata L.), a vital legume in Asia with significant nutritional benefits, is highly susceptible to Cercospora leaf spot (CLS) caused by Cercospora canescens, leading to significant yield losses. As an alternative to chemical fungicides, bio-priming with rhizobacteria can enhance plant resistance. This study explores the potential of Bradyrhizobium sp. strain DOA9 to augment resistance in mung bean against CLS via root priming. The results reveal that short (3 days) and double (17 and 3 days) priming with DOA9 before fungal infection considerably reduces lesion size on infected leaves by activating defense-related genes, including Pti1, Pti6, EDS1, NDR1, PR-1, PR-2, Prx, and CHS, or by suppressing the inhibition of PR-5 and enhancing peroxidase (POD) activity in leaves. Interestingly, the Type 3 secretion system (T3SS) of DOA9 may play a role in establishing resistance in V. radiata CN72. These findings suggest that DOA9 primes V. radiata CN72′s defense mechanisms, offering an effective bio-priming strategy to alleviate CLS. Hence, our insights propose the potential use of DOA9 as a bio-priming agent to manage CLS in V. radiata CN72, providing a sustainable alternative to chemical fungicide applications.
