Generation of a human control PBMC derived iPS cell line TUSMi001-A from a healthy male donor of Han Chinese genetic background

Ying Wang; Yue Zhang; Jing Zhang; Jing Lu; Chaowen Yang; Jian Zhao; Gang Li; Zhongmin Liu; Ying Lei

doi:10.1016/j.scr.2017.10.006

Stem Cell Research (Dec 2017)

Generation of a human control PBMC derived iPS cell line TUSMi001-A from a healthy male donor of Han Chinese genetic background

Ying Wang,
Yue Zhang,
Jing Zhang,
Jing Lu,
Chaowen Yang,
Jian Zhao,
Gang Li,
Zhongmin Liu,
Ying Lei

Affiliations

Ying Wang: Stem Cell Core Facility, Translational Medical Center for Stem Cell Therapy, Shanghai East Hospital, Tongji University School of Medicine, Shanghai, China
Yue Zhang: Department of Neurology, Shanghai East Hospital, Tongji University School of Medicine, Shanghai, China
Jing Zhang: Department of Neurology, Shanghai East Hospital, Tongji University School of Medicine, Shanghai, China
Jing Lu: Department of Neurology, Shanghai East Hospital, Tongji University School of Medicine, Shanghai, China
Chaowen Yang: IxCell Biotechnology Co., Ltd, Shanghai, China
Jian Zhao: Stem Cell Core Facility, Translational Medical Center for Stem Cell Therapy, Shanghai East Hospital, Tongji University School of Medicine, Shanghai, China
Gang Li: Department of Neurology, Shanghai East Hospital, Tongji University School of Medicine, Shanghai, China
Zhongmin Liu: Department of Cardiac Surgery, Shanghai East Hospital, Tongji University School of Medicine, Shanghai, China
Ying Lei: Stem Cell Core Facility, Translational Medical Center for Stem Cell Therapy, Shanghai East Hospital, Tongji University School of Medicine, Shanghai, China

DOI: https://doi.org/10.1016/j.scr.2017.10.006
Journal volume & issue: Vol. 25, no. C
pp. 22 – 25

Abstract

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A 59-year old healthy man of Han Chinese genetic background donated his peripheral blood mononuclear cells (PBMC). The non-integrating episomal vector system was used to reprogram his PBMCs with the human OSKM (Oct4, Sox2, Kl4 and c-Myc) transcription factors. The pluripotency of transgene-free iPSCs was confirmed by immunocytochemistry for pluripotency markers and by the ability of the iPSCs to differentiate spontaneously into 3 germ layers in vitro. In addition, the iPSC line displayed a normal karyotype. In the studies of disease mechanism, the iPSC line can be used as a control.

Published in Stem Cell Research

ISSN: 1873-5061 (Print); 1876-7753 (Online)
Publisher: Elsevier
Country of publisher: Netherlands
LCC subjects: Science: Biology (General)
Website: https://www.journals.elsevier.com/stem-cell-research

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