Integrated regulatory network in Pseudomonas syringae reveals dynamics of virulence
Xiaolong Shao,
Miaomiao Tan,
Yingpeng Xie,
Chunyan Yao,
Tingting Wang,
Hao Huang,
Yingchao Zhang,
Yiqing Ding,
Jingui Liu,
Liangliang Han,
Canfeng Hua,
Xin Wang,
Xin Deng
Affiliations
Xiaolong Shao
Department of Biomedical Sciences, City University of Hong Kong, Kowloon Tong, Hong Kong SAR, China
Miaomiao Tan
Department of Biomedical Sciences, City University of Hong Kong, Kowloon Tong, Hong Kong SAR, China
Yingpeng Xie
Department of Biomedical Sciences, City University of Hong Kong, Kowloon Tong, Hong Kong SAR, China
Chunyan Yao
Department of Biomedical Sciences, City University of Hong Kong, Kowloon Tong, Hong Kong SAR, China
Tingting Wang
Department of Biomedical Sciences, City University of Hong Kong, Kowloon Tong, Hong Kong SAR, China
Hao Huang
Department of Biomedical Sciences, City University of Hong Kong, Kowloon Tong, Hong Kong SAR, China
Yingchao Zhang
Department of Biomedical Sciences, City University of Hong Kong, Kowloon Tong, Hong Kong SAR, China
Yiqing Ding
Department of Biomedical Sciences, City University of Hong Kong, Kowloon Tong, Hong Kong SAR, China
Jingui Liu
Department of Biomedical Sciences, City University of Hong Kong, Kowloon Tong, Hong Kong SAR, China
Liangliang Han
Department of Biomedical Sciences, City University of Hong Kong, Kowloon Tong, Hong Kong SAR, China
Canfeng Hua
Department of Biomedical Sciences, City University of Hong Kong, Kowloon Tong, Hong Kong SAR, China
Xin Wang
Department of Biomedical Sciences, City University of Hong Kong, Kowloon Tong, Hong Kong SAR, China; Shenzhen Research Institute, City University of Hong Kong, Shenzhen 518057, China; Corresponding author
Xin Deng
Department of Biomedical Sciences, City University of Hong Kong, Kowloon Tong, Hong Kong SAR, China; Shenzhen Research Institute, City University of Hong Kong, Shenzhen 518057, China; Corresponding author
Summary: Pseudomonas syringae, a Gram-negative plant pathogen, expresses multitudinous transcriptional regulators to control the type III secretion system (T3SS) and response to diverse environmental challenges. Although the mechanisms of virulence-associated regulators of P. syringae have been studied for decades, the overall crosstalk underlying these regulators is still elusive. Here, we identify five T3SS regulators (EnvZ-OmpR, CbrAB2, PhoPQ, PilRS, and MgrA), and find that the two-component systems EnvZ-OmpR and CbrAB2 negatively regulate the T3SS. To elucidate crosstalk between 16 virulence-associated regulators in P. syringae, we map an online intricate network called “PSRnet” (Pseudomonas syringae regulatory network) by combining the differentially expressed genes (DEGs) of these 16 regulators by RNA sequencing (RNA-seq) and their binding loci by chromatin immunoprecipitation sequencing (ChIP-seq). Consequently, we identify 238 and 153 functional genes involved in the T3SS and other virulence-related pathways in KB and MM media, respectively. Our results provide insights into the mechanism of plant infections caused by P. syringae.