3D Propolis-Sodium Alginate Scaffolds: Influence on Structural Parameters, Release Mechanisms, Cell Cytotoxicity and Antibacterial Activity
Kubra Aranci,
Muhammet Uzun,
Sena Su,
Sumeyye Cesur,
Songul Ulag,
Al Amin,
Mehmet Mucahit Guncu,
Burak Aksu,
Sevgi Kolayli,
Cem Bulent Ustundag,
Jorge Carvalho Silva,
Denisa Ficai,
Anton Ficai,
Oguzhan Gunduz
Affiliations
Kubra Aranci
Center for Nanotechnology & Biomaterials Application and Research (NBUAM), Marmara University, 34722 Istanbul, Turkey
Muhammet Uzun
Center for Nanotechnology & Biomaterials Application and Research (NBUAM), Marmara University, 34722 Istanbul, Turkey
Sena Su
Center for Nanotechnology & Biomaterials Application and Research (NBUAM), Marmara University, 34722 Istanbul, Turkey
Sumeyye Cesur
Center for Nanotechnology & Biomaterials Application and Research (NBUAM), Marmara University, 34722 Istanbul, Turkey
Songul Ulag
Center for Nanotechnology & Biomaterials Application and Research (NBUAM), Marmara University, 34722 Istanbul, Turkey
Al Amin
Department of Textile Engineering, Faculty of Technology, Marmara University, 34722 Istanbul, Turkey
Mehmet Mucahit Guncu
Institute of Health Sciences, Department of Microbiology, Marmara University, Maltepe, 34854 Istanbul, Turkey
Burak Aksu
Department of Medical Microbiology, School of Medicine, Marmara University, Maltepe, 34854 Istanbul, Turkey
Sevgi Kolayli
Department of Chemistry, Faculty of Sciences, Karadeniz Technical University, 61080 Trabzon, Turkey
Cem Bulent Ustundag
Department of Bioengineering, Faculty of Chemical and Metallurgical Engineering, Yıldız Technical University, 34220 Istanbul, Turkey
Jorge Carvalho Silva
Departamento de Física and CENIMAT/i3N, Faculdade de Ciências e Tecnologia, Universidade Nova de Lisboa, 2829-516 Caparica, Portugal
Denisa Ficai
Department of Inorganic Chemistry, Physical Chemistry and Electrochemistry, Faculty of Applied Chemistry and Materials Science, University POLITEHNICA of Bucharest, Gh Polizu Street 1-7, 011061 Bucharest, Romania
Anton Ficai
National Centre for Micro- and Nanomaterials, University POLITEHNICA of Bucharest, Independentei St. 313, 060042 Bucharest, Romania
Oguzhan Gunduz
Center for Nanotechnology & Biomaterials Application and Research (NBUAM), Marmara University, 34722 Istanbul, Turkey
In this study, the main aim was to fabricate propolis (Ps)-containing wound dressing patches using 3D printing technology. Different combinations and structures of propolis (Ps)-incorporated sodium alginate (SA) scaffolds were developed. The morphological studies showed that the porosity of developed scaffolds was optimized when 20% (v/v) of Ps was added to the solution. The pore sizes decreased by increasing Ps concentration up to a certain level due to its adhesive properties. The mechanical, swelling-degradation (weight loss) behaviors, and Ps release kinetics were highlighted for the scaffold stability. An antimicrobial assay was employed to test and screen antimicrobial behavior of Ps against Escherichia coli and Staphylococcus aureus strains. The results show that the Ps-added scaffolds have an excellent antibacterial activity because of Ps compounds. An in vitro cytotoxicity test was also applied on the scaffold by using the extract method on the human dermal fibroblasts (HFFF2) cell line. The 3D-printed SA–Ps scaffolds are very useful structures for wound dressing applications.
