Isolation and Identification of Multidrug-Resistant <i>Klebsiella pneumoniae</i> Clones from the Hospital Environment
María Guadalupe Córdova-Espinoza,
Silvia Giono-Cerezo,
Erika Gabriela Sierra-Atanacio,
Alejandro Escamilla-Gutiérrez,
Eduardo Carrillo-Tapia,
Laura Isabel Carrillo-Vázquez,
Felipe Mendoza-Pérez,
Martha Leyte-Lugo,
Raquel González-Vázquez,
Lino Mayorga-Reyes,
Rosa González-Vázquez
Affiliations
María Guadalupe Córdova-Espinoza
Escuela Nacional de Ciencias Biológicas, Instituto Politécnico Nacional, Departamento de Microbiología, Prolongación de Carpio y Plan de Ayala S/N, Col. Casco de Santo Tomas, Alcaldía Miguel Hidalgo, Mexico City 11340, Mexico
Silvia Giono-Cerezo
Escuela Nacional de Ciencias Biológicas, Instituto Politécnico Nacional, Departamento de Microbiología, Prolongación de Carpio y Plan de Ayala S/N, Col. Casco de Santo Tomas, Alcaldía Miguel Hidalgo, Mexico City 11340, Mexico
Erika Gabriela Sierra-Atanacio
Escuela Nacional de Ciencias Biológicas, Instituto Politécnico Nacional, Departamento de Microbiología, Prolongación de Carpio y Plan de Ayala S/N, Col. Casco de Santo Tomas, Alcaldía Miguel Hidalgo, Mexico City 11340, Mexico
Alejandro Escamilla-Gutiérrez
Escuela Nacional de Ciencias Biológicas, Instituto Politécnico Nacional, Departamento de Microbiología, Prolongación de Carpio y Plan de Ayala S/N, Col. Casco de Santo Tomas, Alcaldía Miguel Hidalgo, Mexico City 11340, Mexico
Eduardo Carrillo-Tapia
Colegio de Ciencias y Humanidades, Universidad Autónoma de la Ciudad de México, Avenida de la Corona 320, Col. Loma de la Palma, Alcaldia Gustavo a Madero, Mexico City 07160, Mexico
Laura Isabel Carrillo-Vázquez
Posgrado en Ciencia Genómicas, Universidad Autónoma de la Ciudad de México, San Lorenzo 290, Col. Del Valle, Alcaldía Benito Juárez, Mexico City 03130, Mexico
Felipe Mendoza-Pérez
Laboratorio de Biotecnología, Departamento de Sistemas Biológicos, Universidad Autónoma Metropolitana Unidad Xochimilco, Calzada del Hueso 1100, Col. Villa Quietud, Alcaldía Coyoacán, Mexico City 04960, Mexico
Martha Leyte-Lugo
Laboratorio de Biotecnología, Departamento de Sistemas Biológicos, CONACYT-Universidad Autónoma Metropolitana Unidad Xochimilco, Calzada del Hueso 1100, Col. Villa Quietud, Alcaldía Coyoacán, Mexico City 04960, Mexico
Raquel González-Vázquez
Laboratorio de Biotecnología, Departamento de Sistemas Biológicos, CONACYT-Universidad Autónoma Metropolitana Unidad Xochimilco, Calzada del Hueso 1100, Col. Villa Quietud, Alcaldía Coyoacán, Mexico City 04960, Mexico
Lino Mayorga-Reyes
Laboratorio de Biotecnología, Departamento de Sistemas Biológicos, Universidad Autónoma Metropolitana Unidad Xochimilco, Calzada del Hueso 1100, Col. Villa Quietud, Alcaldía Coyoacán, Mexico City 04960, Mexico
Rosa González-Vázquez
Escuela Nacional de Ciencias Biológicas, Instituto Politécnico Nacional, Departamento de Microbiología, Prolongación de Carpio y Plan de Ayala S/N, Col. Casco de Santo Tomas, Alcaldía Miguel Hidalgo, Mexico City 11340, Mexico
Global dispersion, hospital outbreaks, and lineage relationships between emerging antibiotic-resistant strains such as Klebsiella pneumoniae are of public health interest. This study aimed to isolate and identify K. pneumoniae clones from third-level healthcare hospitals in Mexico to establish their multidrug-resistant phenotype, phylogeny, and prevalence. Biological and abiotic surface samples were used to isolate K. pneumoniae strains and to test their antibiotic susceptibility to classify them. The housekeeping genes: gapA, InfB, mdh, pgi, phoE, ropB, and tonB were used for multilocus sequence typing (MLST). Phylogenetic networks were constructed with 48 strains. Isolated strains (93) were mainly from urine and blood, 96% were resistant to ampicillin as expected, 60% were extended-spectrum β-lactamases (ESBL), 98% were susceptible to ertapenem and meropenem and 99% were susceptible to imipenem, 46% were multi-drug resistant (MDR), 17% were extensively-drug resistant (XDR), 1% were pan-drug resistant (PDR), and 36% were not classified. The tonB, mdh, and phoE genes were the most variable, and the InfB gene showed positive selection. The most prevalent sequence types (STs) were ST551 (six clones), ST405 (six clones), ST1088 (four clones), ST25 (four clones), ST392 (three clones), and ST36 (two clones). ST706 was PDR, and ST1088 clones were MDR; neither of these STs has been reported in Mexico. The strains analyzed were from different hospitals and locations; thus, it is important to maintain antibiotic surveillance and avoid clone dissemination to prevent outbreaks, adaptation to antibiotics, and the transmission of antibiotic resistance.
