The <i>CDPK</i> Gene Family in Mustard (<i>Brassica juncea</i> L.): Genome-Wide Identification and Expression Analysis under Cold Stress
Haibo Li,
Hao Wu,
Weifeng Huang,
Jiaxian Liu,
Jiaxin Deng,
Chuanhong Li,
Cui Mao,
Yang Zhang,
Yukun Wang,
Jie Zheng
Affiliations
Haibo Li
Guangdong Provincial Key Laboratory of Utilization and Conservation of Food and Medicinal Resources in Northern Region, Shaoguan University, Shaoguan 512000, China
Hao Wu
Guangdong Provincial Key Laboratory of Utilization and Conservation of Food and Medicinal Resources in Northern Region, Shaoguan University, Shaoguan 512000, China
Weifeng Huang
School of Biology and Agriculture, Shaoguan University, Shaoguan 512000, China
Jiaxian Liu
School of Biology and Agriculture, Shaoguan University, Shaoguan 512000, China
Jiaxin Deng
School of Biology and Agriculture, Shaoguan University, Shaoguan 512000, China
Chuanhong Li
School of Biology and Agriculture, Shaoguan University, Shaoguan 512000, China
Cui Mao
School of Biology and Agriculture, Shaoguan University, Shaoguan 512000, China
Yang Zhang
Guangdong Provincial Key Laboratory of Utilization and Conservation of Food and Medicinal Resources in Northern Region, Shaoguan University, Shaoguan 512000, China
Yukun Wang
Guangdong Provincial Key Laboratory of Utilization and Conservation of Food and Medicinal Resources in Northern Region, Shaoguan University, Shaoguan 512000, China
Jie Zheng
Guangdong Provincial Key Laboratory of Utilization and Conservation of Food and Medicinal Resources in Northern Region, Shaoguan University, Shaoguan 512000, China
The CDPK family genes play crucial roles in signal transduction pathways during plant development and stress response. In this study, we comprehensively analyzed the CDPK family genes in mustard (Brassica juncea L.), resulting in the identification of 101 genes (BjuCDPK1–101) located on chromosomes AA_Chr01 to BB_Chr08. RNA-seq analysis showed that most BjuCDPK genes were predominantly expressed in the root and flower bud, suggesting their organ-specific expression patterns. Furthermore, the expression levels of most BjuCDPK genes were significantly altered by cold stress. The promoter regions of BjuCDPK genes had abundant cis-acting elements related to hormones and stress. The interaction network analysis predicted that BjuCDPK proteins respond to abiotic stress probably via interaction with ABF1, ABF4, and/or DI19. Our findings provide valuable information for further the investigation of the cold stress adaption of mustard via the CDPK signaling pathway.
