Xin yixue (Mar 2022)
Role of heterotopic heart transplantation in establishment of rat models with unloading the infarcted hearts
Abstract
Objective To investigate the highlights of establishing a rat model of mimicking left venticular assist device （LVAD） by unloading rat infarcted hearts. Methods 29 adult Lewis rats were ligated the left anterior discending arteries to shut the blood supply of myocardium to establish the infarcted heart failure rat models. Electrocardiogram （ECG） was performed to screen the rat models with heart failure. 24 heart failure rats were randomly divided into the unloading group （n = 14） and heart failure group （n = 10）. Heart transplantation was not performed in the heart failure group. In the unloading group, the hearts of heart failure rats were prepared for heterotopic cardiac transplantation into the inferior vena cava and abdominal aorta of the recipient rats to establish the left ventricular unloading models. In the blank control group （n = 8）, the rats were intervened with open surgery alone. The remaining interventions were similar to those in the other two groups. At 2 weeks after cardiac transplantation, the left ventricular end-diastolic pressure （LVEDP）, maximum left ventricular pressure rising/falling rate （±dp/dtmax） and left ventricular systolic pressure （LVSP） were measured. Histological changes of the rat hearts were observed. Results The success rate of heart failure rat models after ischemic cardiomyopathy was 82.76% （24/29）. The survival rate of heart failure rats after cardiac unloading was 71.43% （10/14）. Compared with the heart failure group, the left ventricular developed pressure （LVDP） was significantly elevated （47.85±0.94 vs. 35.94±0.95,F = 250.993, P < 0.05） and the ±dp/dtmax was significantly increased （1177.85±61.03 vs. 841.64±40.26,F = 942.304,P < 0.05） in the unloading group. Conclusion The rat models with heart failure can be established by myocardial infarction and subsequently receive heterotopic heart transplantation, which offers an economical and reproducible model of ventricular unloading.
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