Molecular recognition of the native HIV-1 MPER revealed by STED microscopy of single virions

Pablo Carravilla; Jakub Chojnacki; Edurne Rujas; Sara Insausti; Eneko Largo; Dominic Waithe; Beatriz Apellaniz; Taylor Sicard; Jean-Philippe Julien; Christian Eggeling; José L. Nieva

doi:10.1038/s41467-018-07962-9

Nature Communications (Jan 2019)

Molecular recognition of the native HIV-1 MPER revealed by STED microscopy of single virions

Pablo Carravilla,
Jakub Chojnacki,
Edurne Rujas,
Sara Insausti,
Eneko Largo,
Dominic Waithe,
Beatriz Apellaniz,
Taylor Sicard,
Jean-Philippe Julien,
Christian Eggeling,
José L. Nieva

Affiliations

Pablo Carravilla: Biofisika Institute (CSIC, UPV/EHU) and Department of Biochemistry and Molecular Biology, University of the Basque Country (UPV/EHU)
Jakub Chojnacki: MRC Human Immunology Unit, Weatherall Institute of Molecular Medicine, University of Oxford
Edurne Rujas: Biofisika Institute (CSIC, UPV/EHU) and Department of Biochemistry and Molecular Biology, University of the Basque Country (UPV/EHU)
Sara Insausti: Biofisika Institute (CSIC, UPV/EHU) and Department of Biochemistry and Molecular Biology, University of the Basque Country (UPV/EHU)
Eneko Largo: Biofisika Institute (CSIC, UPV/EHU) and Department of Biochemistry and Molecular Biology, University of the Basque Country (UPV/EHU)
Dominic Waithe: MRC Human Immunology Unit, Weatherall Institute of Molecular Medicine, University of Oxford
Beatriz Apellaniz: Biofisika Institute (CSIC, UPV/EHU) and Department of Biochemistry and Molecular Biology, University of the Basque Country (UPV/EHU)
Taylor Sicard: Program in Molecular Medicine, The Hospital for Sick Children Research Institute
Jean-Philippe Julien: Program in Molecular Medicine, The Hospital for Sick Children Research Institute
Christian Eggeling: MRC Human Immunology Unit, Weatherall Institute of Molecular Medicine, University of Oxford
José L. Nieva: Biofisika Institute (CSIC, UPV/EHU) and Department of Biochemistry and Molecular Biology, University of the Basque Country (UPV/EHU)

DOI: https://doi.org/10.1038/s41467-018-07962-9
Journal volume & issue: Vol. 10, no. 1
pp. 1 – 11

Abstract

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The Membrane-Proximal External Region (MPER) of the HIV Env gp41 subunit is a target for broadly neutralizing antibodies. Here, the authors apply super-resolution stimulated emission depletion (STED) microscopy on single virions and provide insights into how the MPER epitope is recognized.

Published in Nature Communications

ISSN: 2041-1723 (Online)
Publisher: Nature Portfolio
Country of publisher: United Kingdom
LCC subjects: Science
Website: https://www.nature.com/ncomms/

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