Biological Procedures Online (Jan 2004)

Serum-Free Cryopreservation of Five Mammalian Cell Lines in Either a Pelleted or Suspended State

  • Corsini Joe,
  • Hacker Christy,
  • Bare Charles

DOI
https://doi.org/10.1251/bpo73
Journal volume & issue
Vol. 6, no. 1
pp. 61 – 66

Abstract

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Herein we have explored two practical aspects of cryopreserving cultured mammalian cells during routine laboratory maintenance. First, we have examined the possibility of using a serum-free, hence more affordable, cryopreservative. Using five mammalian lines (Crandell Feline Kidney, MCF7, A72, WI 38 and NB324K), we found that the serum-free alternative preserves nearly as efficiently as the serum-containing preservatives. Second, we compared cryostorage of those cells in suspended versus a pellet form using both aforementioned cryopreservatives. Under our conditions, cells were in general recovered equally well in a suspended versus a pellet form.

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