Cost-Effective Microsatellite Markers for <i>Banksia integrifolia</i> (Proteaceae)

Abstract

Premise of the study: Microsatellite markers were developed to assess the level of genetic variation and population structure in Banksia integrifolia, a widespread species endemic to eastern Australia. Methods and Results: We used next-generation sequencing approaches to identify and develop 11 polymorphic microsatellite markers with perfect tri- and tetranucleotide repeats. We tested these markers with 71 specimens from three populations. Observed and expected heterozygosities ranged from 0.0 to 0.875 and 0.0 to 0.763, respectively. Conclusions: The developed markers will be valuable for studies of the population structure, mating system, and selection of provenances for restoration projects involving B. integrifolia.

Keywords

• Banksia integrifolia
• coast banksia
• genetic variation
• next-generation sequencing
• Proteaceae
• simple sequence repeat markers