Frontiers in Immunology (Nov 2023)
De novo identification of bacterial antigens of a clinical isolate by combining use of proteosurfaceomics, secretomics, and BacScan technologies
- Jinyue Yang,
- Jinyue Yang,
- Jinyue Yang,
- Xueting Zhang,
- Xueting Zhang,
- Xueting Zhang,
- Junhua Dong,
- Junhua Dong,
- Junhua Dong,
- Qian Zhang,
- Qian Zhang,
- Qian Zhang,
- Erchao Sun,
- Erchao Sun,
- Erchao Sun,
- Cen Chen,
- Cen Chen,
- Cen Chen,
- Zhuangxia Miao,
- Zhuangxia Miao,
- Zhuangxia Miao,
- Yifei Zheng,
- Nan Zhang,
- Pan Tao,
- Pan Tao,
- Pan Tao
Affiliations
- Jinyue Yang
- State Key Laboratory of Agricultural Microbiology, College of Veterinary Medicine, Huazhong Agricultural University, Wuhan, Hubei, China
- Jinyue Yang
- Key Laboratory of Prevention & Control for African Swine Fever and Other Major Pig Diseases, Ministry of Agriculture and Rural Affairs, Cooperative Innovation Center for Sustainable Pig Production, Huazhong Agricultural University, Wuhan, Hubei, China
- Jinyue Yang
- Hubei Hongshan Lab, Wuhan, Hubei, China
- Xueting Zhang
- State Key Laboratory of Agricultural Microbiology, College of Veterinary Medicine, Huazhong Agricultural University, Wuhan, Hubei, China
- Xueting Zhang
- Key Laboratory of Prevention & Control for African Swine Fever and Other Major Pig Diseases, Ministry of Agriculture and Rural Affairs, Cooperative Innovation Center for Sustainable Pig Production, Huazhong Agricultural University, Wuhan, Hubei, China
- Xueting Zhang
- Hubei Hongshan Lab, Wuhan, Hubei, China
- Junhua Dong
- State Key Laboratory of Agricultural Microbiology, College of Veterinary Medicine, Huazhong Agricultural University, Wuhan, Hubei, China
- Junhua Dong
- Key Laboratory of Prevention & Control for African Swine Fever and Other Major Pig Diseases, Ministry of Agriculture and Rural Affairs, Cooperative Innovation Center for Sustainable Pig Production, Huazhong Agricultural University, Wuhan, Hubei, China
- Junhua Dong
- Hubei Hongshan Lab, Wuhan, Hubei, China
- Qian Zhang
- State Key Laboratory of Agricultural Microbiology, College of Veterinary Medicine, Huazhong Agricultural University, Wuhan, Hubei, China
- Qian Zhang
- Key Laboratory of Prevention & Control for African Swine Fever and Other Major Pig Diseases, Ministry of Agriculture and Rural Affairs, Cooperative Innovation Center for Sustainable Pig Production, Huazhong Agricultural University, Wuhan, Hubei, China
- Qian Zhang
- Hubei Hongshan Lab, Wuhan, Hubei, China
- Erchao Sun
- State Key Laboratory of Agricultural Microbiology, College of Veterinary Medicine, Huazhong Agricultural University, Wuhan, Hubei, China
- Erchao Sun
- Key Laboratory of Prevention & Control for African Swine Fever and Other Major Pig Diseases, Ministry of Agriculture and Rural Affairs, Cooperative Innovation Center for Sustainable Pig Production, Huazhong Agricultural University, Wuhan, Hubei, China
- Erchao Sun
- Hubei Hongshan Lab, Wuhan, Hubei, China
- Cen Chen
- State Key Laboratory of Agricultural Microbiology, College of Veterinary Medicine, Huazhong Agricultural University, Wuhan, Hubei, China
- Cen Chen
- Key Laboratory of Prevention & Control for African Swine Fever and Other Major Pig Diseases, Ministry of Agriculture and Rural Affairs, Cooperative Innovation Center for Sustainable Pig Production, Huazhong Agricultural University, Wuhan, Hubei, China
- Cen Chen
- Hubei Hongshan Lab, Wuhan, Hubei, China
- Zhuangxia Miao
- State Key Laboratory of Agricultural Microbiology, College of Veterinary Medicine, Huazhong Agricultural University, Wuhan, Hubei, China
- Zhuangxia Miao
- Key Laboratory of Prevention & Control for African Swine Fever and Other Major Pig Diseases, Ministry of Agriculture and Rural Affairs, Cooperative Innovation Center for Sustainable Pig Production, Huazhong Agricultural University, Wuhan, Hubei, China
- Zhuangxia Miao
- Hubei Hongshan Lab, Wuhan, Hubei, China
- Yifei Zheng
- Veterinary Diagnostic Laboratory, Neixiang Center for Animal Disease Control and Prevention, Nanyang, Henan, China
- Nan Zhang
- Neixiang Animal Health Supervision, Neixiang Animal Husbandry Bureau, Nanyang, Henan, China
- Pan Tao
- State Key Laboratory of Agricultural Microbiology, College of Veterinary Medicine, Huazhong Agricultural University, Wuhan, Hubei, China
- Pan Tao
- Key Laboratory of Prevention & Control for African Swine Fever and Other Major Pig Diseases, Ministry of Agriculture and Rural Affairs, Cooperative Innovation Center for Sustainable Pig Production, Huazhong Agricultural University, Wuhan, Hubei, China
- Pan Tao
- Hubei Hongshan Lab, Wuhan, Hubei, China
- DOI
- https://doi.org/10.3389/fimmu.2023.1274027
- Journal volume & issue
-
Vol. 14
Abstract
BackgroundEmerging infectious diseases pose a significant threat to both human and animal populations. Rapid de novo identification of protective antigens from a clinical isolate and development of an antigen-matched vaccine is a golden strategy to prevent the spread of emerging novel pathogens.MethodsHere, we focused on Actinobacillus pleuropneumoniae, which poses a serious threat to the pig industry, and developed a general workflow by integrating proteosurfaceomics, secretomics, and BacScan technologies for the rapid de novo identification of bacterial protective proteins from a clinical isolate.ResultsAs a proof of concept, we identified 3 novel protective proteins of A. pleuropneumoniae. Using the protective protein HBS1_14 and toxin proteins, we have developed a promising multivalent subunit vaccine against A. pleuropneumoniae.DiscussionWe believe that our strategy can be applied to any bacterial pathogen and has the potential to significantly accelerate the development of antigen-matched vaccines to prevent the spread of an emerging novel bacterial pathogen.
Keywords
- emerging bacterial pathogens
- highly immunogenic proteins
- de novo identification
- vaccine
- A. pleuropneumoniae
