npj Regenerative Medicine (Jun 2022)
Intestinal stem cell aging signature reveals a reprogramming strategy to enhance regenerative potential
- Christian M. Nefzger,
- Thierry Jardé,
- Akanksha Srivastava,
- Jan Schroeder,
- Fernando J. Rossello,
- Katja Horvay,
- Mirsada Prasko,
- Jacob M. Paynter,
- Joseph Chen,
- Chen-Fang Weng,
- Yu B. Y. Sun,
- Xiaodong Liu,
- Eva Chan,
- Nikita Deshpande,
- Xiaoli Chen,
- Y. Jinhua Li,
- Jahnvi Pflueger,
- Rebekah M. Engel,
- Anja S. Knaupp,
- Kirill Tsyganov,
- Susan K. Nilsson,
- Ryan Lister,
- Owen J. L. Rackham,
- Helen E. Abud,
- Jose M. Polo
Affiliations
- Christian M. Nefzger
- Department of Anatomy and Developmental Biology, Monash University
- Thierry Jardé
- Department of Anatomy and Developmental Biology, Monash University
- Akanksha Srivastava
- Australian Research Council Centre of Excellence in Plant Energy Biology, School of Molecular Sciences, The University of Western Australia
- Jan Schroeder
- Department of Anatomy and Developmental Biology, Monash University
- Fernando J. Rossello
- Department of Anatomy and Developmental Biology, Monash University
- Katja Horvay
- Department of Anatomy and Developmental Biology, Monash University
- Mirsada Prasko
- Department of Anatomy and Developmental Biology, Monash University
- Jacob M. Paynter
- Department of Anatomy and Developmental Biology, Monash University
- Joseph Chen
- Department of Anatomy and Developmental Biology, Monash University
- Chen-Fang Weng
- Department of Anatomy and Developmental Biology, Monash University
- Yu B. Y. Sun
- Department of Anatomy and Developmental Biology, Monash University
- Xiaodong Liu
- Department of Anatomy and Developmental Biology, Monash University
- Eva Chan
- Department of Anatomy and Developmental Biology, Monash University
- Nikita Deshpande
- Institute for Molecular Bioscience, University of Queensland
- Xiaoli Chen
- Institute for Molecular Bioscience, University of Queensland
- Y. Jinhua Li
- Department of Anatomy and Developmental Biology, Monash University
- Jahnvi Pflueger
- Australian Research Council Centre of Excellence in Plant Energy Biology, School of Molecular Sciences, The University of Western Australia
- Rebekah M. Engel
- Department of Anatomy and Developmental Biology, Monash University
- Anja S. Knaupp
- Department of Anatomy and Developmental Biology, Monash University
- Kirill Tsyganov
- Monash Bioinformatics Platform, Monash University
- Susan K. Nilsson
- Australian Regenerative Medicine Institute, Monash University
- Ryan Lister
- Australian Research Council Centre of Excellence in Plant Energy Biology, School of Molecular Sciences, The University of Western Australia
- Owen J. L. Rackham
- Program in Cardiovascular and Metabolic Disorders, Duke-National University of Singapore Medical School
- Helen E. Abud
- Department of Anatomy and Developmental Biology, Monash University
- Jose M. Polo
- Department of Anatomy and Developmental Biology, Monash University
- DOI
- https://doi.org/10.1038/s41536-022-00226-7
- Journal volume & issue
-
Vol. 7,
no. 1
pp. 1 – 8
Abstract
Abstract The impact of aging on intestinal stem cells (ISCs) has not been fully elucidated. In this study, we identified widespread epigenetic and transcriptional alterations in old ISCs. Using a reprogramming algorithm, we identified a set of key transcription factors (Egr1, Irf1, FosB) that drives molecular and functional differences between old and young states. Overall, by dissecting the molecular signature of aged ISCs, our study identified transcription factors that enhance the regenerative capacity of ISCs.
