International Journal of Molecular Sciences (Nov 2022)

The Cysteine Protease Giardipain-1 from <i>Giardia duodenalis</i> Contributes to a Disruption of Intestinal Homeostasis

  • Rodrigo Quezada-Lázaro,
  • Yessica Vázquez-Cobix,
  • Rocío Fonseca-Liñán,
  • Porfirio Nava,
  • Daniel Dimitri Hernández-Cueto,
  • Carlos Cedillo-Peláez,
  • Yolanda López-Vidal,
  • Sara Huerta-Yepez,
  • M. Guadalupe Ortega-Pierres

DOI
https://doi.org/10.3390/ijms232113649
Journal volume & issue
Vol. 23, no. 21
p. 13649

Abstract

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In giardiasis, diarrhoea, dehydration, malabsorption, weight loss and/or chronic inflammation are indicative of epithelial barrier dysfunction. However, the pathogenesis of giardiasis is still enigmatic in many aspects. Here, we show evidence that a cysteine protease of Giardia duodenalis called giardipain-1, contributes to the pathogenesis of giardiasis induced by trophozoites of the WB strain. In an experimental system, we demonstrate that purified giardipain-1 induces apoptosis and extrusion of epithelial cells at the tips of the villi in infected jirds (Meriones unguiculatus). Moreover, jird infection with trophozoites expressing giardipain-1 resulted in intestinal epithelial damage, cellular infiltration, crypt hyperplasia, goblet cell hypertrophy and oedema. Pathological alterations were more pronounced when jirds were infected intragastrically with Giardia trophozoites that stably overexpress giardipain-1. Furthermore, Giardia colonization in jirds results in a chronic inflammation that could relate to the dysbiosis triggered by the protist. Taken together, these results reveal that giardipain-1 plays a key role in the pathogenesis of giardiasis.

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