Frontiers in Aging Neuroscience (May 2021)
Circular RNA circDUS2 Is a Potential Biomarker for Intracranial Aneurysm
- Xin Chen,
- Xin Chen,
- Xin Chen,
- Xin Chen,
- Shuzhe Yang,
- Shuzhe Yang,
- Shuzhe Yang,
- Shuzhe Yang,
- Junhua Yang,
- Junhua Yang,
- Junhua Yang,
- Junhua Yang,
- Qingyuan Liu,
- Qingyuan Liu,
- Qingyuan Liu,
- Qingyuan Liu,
- Maogui Li,
- Maogui Li,
- Maogui Li,
- Maogui Li,
- Jun Wu,
- Jun Wu,
- Jun Wu,
- Jun Wu,
- Hao Wang,
- Hao Wang,
- Hao Wang,
- Hao Wang,
- Shuo Wang,
- Shuo Wang,
- Shuo Wang,
- Shuo Wang
Affiliations
- Xin Chen
- Department of Neurosurgery, Beijing Tiantan Hospital, Capital Medical University, Beijing, China
- Xin Chen
- China National Clinical Research Center for Neurological Diseases, Beijing, China
- Xin Chen
- Center of Stroke, Beijing Institute for Brain Disorders, Beijing, China
- Xin Chen
- Beijing Key Laboratory of Translational Medicine for Cerebrovascular Diseases, Beijing, China
- Shuzhe Yang
- Department of Neurosurgery, Beijing Tiantan Hospital, Capital Medical University, Beijing, China
- Shuzhe Yang
- China National Clinical Research Center for Neurological Diseases, Beijing, China
- Shuzhe Yang
- Center of Stroke, Beijing Institute for Brain Disorders, Beijing, China
- Shuzhe Yang
- Beijing Key Laboratory of Translational Medicine for Cerebrovascular Diseases, Beijing, China
- Junhua Yang
- Department of Neurosurgery, Beijing Tiantan Hospital, Capital Medical University, Beijing, China
- Junhua Yang
- China National Clinical Research Center for Neurological Diseases, Beijing, China
- Junhua Yang
- Center of Stroke, Beijing Institute for Brain Disorders, Beijing, China
- Junhua Yang
- Beijing Key Laboratory of Translational Medicine for Cerebrovascular Diseases, Beijing, China
- Qingyuan Liu
- Department of Neurosurgery, Beijing Tiantan Hospital, Capital Medical University, Beijing, China
- Qingyuan Liu
- China National Clinical Research Center for Neurological Diseases, Beijing, China
- Qingyuan Liu
- Center of Stroke, Beijing Institute for Brain Disorders, Beijing, China
- Qingyuan Liu
- Beijing Key Laboratory of Translational Medicine for Cerebrovascular Diseases, Beijing, China
- Maogui Li
- Department of Neurosurgery, Beijing Tiantan Hospital, Capital Medical University, Beijing, China
- Maogui Li
- China National Clinical Research Center for Neurological Diseases, Beijing, China
- Maogui Li
- Center of Stroke, Beijing Institute for Brain Disorders, Beijing, China
- Maogui Li
- Beijing Key Laboratory of Translational Medicine for Cerebrovascular Diseases, Beijing, China
- Jun Wu
- Department of Neurosurgery, Beijing Tiantan Hospital, Capital Medical University, Beijing, China
- Jun Wu
- China National Clinical Research Center for Neurological Diseases, Beijing, China
- Jun Wu
- Center of Stroke, Beijing Institute for Brain Disorders, Beijing, China
- Jun Wu
- Beijing Key Laboratory of Translational Medicine for Cerebrovascular Diseases, Beijing, China
- Hao Wang
- Department of Neurosurgery, Beijing Tiantan Hospital, Capital Medical University, Beijing, China
- Hao Wang
- China National Clinical Research Center for Neurological Diseases, Beijing, China
- Hao Wang
- Center of Stroke, Beijing Institute for Brain Disorders, Beijing, China
- Hao Wang
- Beijing Key Laboratory of Translational Medicine for Cerebrovascular Diseases, Beijing, China
- Shuo Wang
- Department of Neurosurgery, Beijing Tiantan Hospital, Capital Medical University, Beijing, China
- Shuo Wang
- China National Clinical Research Center for Neurological Diseases, Beijing, China
- Shuo Wang
- Center of Stroke, Beijing Institute for Brain Disorders, Beijing, China
- Shuo Wang
- Beijing Key Laboratory of Translational Medicine for Cerebrovascular Diseases, Beijing, China
- DOI
- https://doi.org/10.3389/fnagi.2021.632448
- Journal volume & issue
-
Vol. 13
Abstract
Background: CircRNAs have been found to play a crucial role in the pathological process of various kinds of diseases. However, the role of circRNAs in the formation and rupture of intracranial aneurysm is still unknown.Methods: Differentially expressed circRNAs profiles between superficial temporal arteries (n = 5) and intracranial aneurysms (n = 5) were analyzed using the Arraystar human circRNAs microarray. Quantitative real-time PCR was utilized to validate the differential expression of circDUS2. Fluorescence in situ hybridization (FISH) was meant for the location of circDUS2 in human brain vascular smooth muscle cell (HBVSMC). Structural analysis was used to speculate on the function of circDUS2.Results: Five hundred forty-three upregulated and 397 downregulated significantly in intracranial aneurysm as compared to superficial temporal arteries. Quantitative real-time PCR verified the elevated expression of the upregulated circDUS2. The FISH test revealed that circDUS2 is located in the cytoplasm of brain vascular smooth muscle cells.Conclusion: This study showed differential expression data of circRNAs between superficial temporal artery and intracranial aneurysm and revealed that circDUS2 is a potential molecular marker for intracranial aneurysm.
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