Tetranortriterpenes and Limonoids from the Roots of Aphanamixis polystachya
Ching-Jie Lin,
I-Wen Lo,
Yu-Chi Lin,
Shun-Ying Chen,
Ching-Te Chien,
Yao-Haur Kuo,
Tsong-Long Hwang,
Shorong-Shii Liou,
Ya-Ching Shen
Affiliations
Ching-Jie Lin
School of Pharmacy, College of Medicine, National Taiwan University, No. 33, Linsen S. Rd., Zhongzheng Dist., Taipei 10050, Taiwan
I-Wen Lo
School of Pharmacy, College of Medicine, National Taiwan University, No. 33, Linsen S. Rd., Zhongzheng Dist., Taipei 10050, Taiwan
Yu-Chi Lin
Department of Life Sciences, National Cheng Kung University, No. 1, University Road, Tainan 701, Taiwan
Shun-Ying Chen
Taiwan Forestry Research Institute, Council of Agriculture, Executive Yuan, No. 53, Nanhai Rd., Zhongzheng Dist., Taipei 10066, Taiwan
Ching-Te Chien
Taiwan Forestry Research Institute, Council of Agriculture, Executive Yuan, No. 53, Nanhai Rd., Zhongzheng Dist., Taipei 10066, Taiwan
Yao-Haur Kuo
National Research Institute of Chinese Medicine, Ministry of Health and Welfare, No. 155-1, Sec. 2, Linong St., Beitou District, Taipei 11221, Taiwan
Tsong-Long Hwang
Graduate Institute of Natural Products, School of Traditional Medicine, College of Medicine, and Chinese Herbal Medicine Research Team, Healthy Aging Research Center, Chang Gung University, Taoyuan 33302, Taiwan
Shorong-Shii Liou
Department of Pharmacy, Tajen University, No. 20, Weixin Rd., Yanpu Township, Pingtung 90741, Taiwan
Ya-Ching Shen
School of Pharmacy, College of Medicine, National Taiwan University, No. 33, Linsen S. Rd., Zhongzheng Dist., Taipei 10050, Taiwan
Phytochemical investigation of the acetone extract from the roots of Aphanamixis polystachya resulted in isolation of four new tetranortriterpenes (1–4) in addition to one protolimonoid (methyl-1ξ,7R-diacetoxy-23R,25-dihydroxy-20S,24R-21,24-epoxy-3,4-seco-apotirucall-4(28),14(15)-diene-3-oate (5)), five known limonoids (rohituka 3 (6), rohituka 7 (7), nymania 1 (8), rubrin G (9), prieurianin (10)) and a steroid (2,3-dihydroxy-5-pregnan-16-one (11)). Their structures were determined by spectroscopic analyses, including 2D-NMR (COSY, HMQC, HMBC, and NOESY) and high-resolution electrospray ionization mass spectrometry (HRESIMS). Cytotoxic and anti-inflammatory activities of these compounds were evaluated. Compounds 4 and 5 showed significant inhibition against superoxide generation and elastase release by human neutrophils in response to (formyl-l-methionyl-l-leucyl-l-phenylalanine/cytochalasin B) (FMLP/CB).
