REACTION OF PERIPHERAL BLOOD LYMPHOCYTES OF ADOLESCENTS WITH DEPRESSION TO CHEMICAL MUTAGEN IN VITRO

Abstract

The study of chromosomal disorder incidence allows us to assess influence of environmental factors on human body that is due to the fact that the level of spontaneous chromosomal mutagenesis in human population is a relatively constant value used to assess susceptibility of human body to the effects of mutagenic agents. Cytogenetic analysis was carried out in accordance with international requirements in 24 adolescents (12 girls and 12 boys) aged 14 to 18 of both sexes with depression. Additional mutagenic effect of mitomycin C on blood lymphocytes was used to determine possible latent chromosomal instability in vitro. Statistical processing of obtained data was carried out using Excel software package. Student’s t-test was used to determine the probability of differences between groups. Spontaneous level of chromosomal disorders in intact cultures of blood lymphocytes of adolescents with depression constituted 8.3 and it increased up to 17.5 per 100 metaphase plates after the effect of mitomycin C on blood lymphocytes of patients. Frequency of aberrations of chromatid type increased 1.5 times, chromosome type – 2.6 times. Both before and after mutagen effect on blood lymphocytes of patients, single acentric fragments prevailed among chromatid type aberrations and paired acentric fragments and dicentric chromosomes prevailed among chromosome type aberrations. Spontaneous individual frequency of chromosome aberrations in groups of girls and boys with depression did not differ and it increased significantly after the effect of chemical mutagen on blood lymphocytes in vitro of patients, mainly due to chromosome aberrations in boys. The cytogenetic effect induced by mitomycin C exceeds mean group level of chromosomal aberrations and corresponds to > 1 in 46.0% of patients and the coefficient of latent chromosomal instability was less than one in 54.0% of patients. Thus, individual somatic chromosome susceptibility to additional mutagenic load in vitro did not depend on sex and influence of mutagenic environmental factors and is genetically determined. We determined the level of spontaneous and induced mutagenesis in blood lymphocytes of patients with depression in vitro, which was twice the spontaneous level of chromosome aberrations after mutagen introduction into the culture mixture that indicates pronounced individual and group latent chromosomal instability in adolescents with depression.

Keywords

• adolescents,depression,mutagen,aberrations,chromosomal instability