Antimicrobial Effect of Two Single File Systems versus One Multiple Rotary Instrumentation on Infected Root Canals

Abstract

Since microorganisms play a fundamental role in the ethology of pulp and periapical diseases, and reduction or elimination of the intracanal population, is a main principles of successful root canal treatment. The aim of this study was the in vitro comparison of bacterial population reduction in root canal by mechanical instrumentation, using two single file systems and multi-file rotary system in root canals infected with Enterococous Faecalis.Materials & Methods:In this experimental laboratory study, the root canals of 48 human mandibular premolar teeth were selected and infected with a culture of E.faecalis for 30 days. Then the specimens were randomly divided into 3 groups (n = 16): Reciproce, Neolix and Mtwo files. Four other teeth that were contaminated and not instrumented, were used as the control group. Samples were taken before (S1) and after instrumentation (S2). Bacterial quantification was performed using quantitive PCR. Statistical analyses were performed using the Mann-Whitney and Kruskal-Wallis tests.Results:Comparing the average number of bacteria before and after preparation, the number of bacteria increased significantly between the three groups of Reciproc, Neolix and MTO after preparation (p value <0.001). However in the control group, there was no significant difference in the number of bacteria before and after preparation (pvalue = 0.144).Conclusion:Single file canal preparation systems are as effective as multifile rotary system in reducingthe bacterial load of canals

Keywords

• enterococcus faecalis
• antimicrobial
• endodontics