Synthesis of Paclitaxel Loaded on Nanoparticles of Archaeosomes and Investigation its Anti-Cancer Properties on Breast Cancer MDA-MB231 cells

Abstract

In this study the effect of Archaeosomal Paclitaxel drug on Breast Cancer cell lines MDA-MB231 cells was evaluated. The experiments were conducted to assess the cytotoxicity of 7 Paclitaxel in free form and compare it with Archaeosomal form of the drug in vitro. To check the size of Archaeosomes made, Zeta sizer device (DLS) was used. The average size of Archaeosom Nanoparticles containing Paclitaxel drugs 430 nm and surface charge -15, the average size of control Archaeosom Nanoparticles without drug 460 nm and surface charge-14 were reported. In both formulations zeta potential was negative. Previous studies have shown negatively charged liposomes have high physical stability, small particle size and high percentage of trapping the drug in the comparison with positively charged. Nano-Archaeosom containing Paclitaxel drug has higher physical stability compared with control Nano-Archaeosom. The effect of Archaeosomal drug containing Paclitaxel and free drug were examined by MTT test. Tests showed the killing effect of Nano-Archaeosome containing Paclitaxel formulation Tumor cells is more than free formulation. In the other word, Nano-Archaeosome containing Paclitaxel is more effective than free Paclitaxel.