Genetics and Molecular Biology (Jan 1998)

A rapid detection method for PAI-1 promoter insertion/deletion polymorphism (4G/5G)

  • Annichino-Bizzacchi Joyce M.,
  • Pugliese Luciana,
  • Arruda Valder R.

Journal volume & issue
Vol. 21, no. 3

Abstract

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Plasminogen activator inhibitor-1 (PAI-1) is an important inhibitor of fibrinolysis, and increased levels of PAI-1 are associated with atheroma and myocardial infarction. A common 4G/5G insertion/deletion polymorphism located in the promoter region of PAI-1 gene has been described associated with PAI-1 activity in plasma levels. Genotyping of this polymorphism is commonly conducted with an allele-specific oligonucleotide melting technique. In the present study, we describe a quick, easy method for genotyping 4G/5G polymorphism in the promoter region of the PAI-1 gene.