Suppression of unwanted CRISPR-Cas9 editing by co-administration of catalytically inactivating truncated guide RNAs

John C. Rose; Nicholas A. Popp; Christopher D. Richardson; Jason J. Stephany; Julie Mathieu; Cindy T. Wei; Jacob E. Corn; Dustin J. Maly; Douglas M. Fowler

doi:10.1038/s41467-020-16542-9

Nature Communications (Jun 2020)

Suppression of unwanted CRISPR-Cas9 editing by co-administration of catalytically inactivating truncated guide RNAs

John C. Rose,
Nicholas A. Popp,
Christopher D. Richardson,
Jason J. Stephany,
Julie Mathieu,
Cindy T. Wei,
Jacob E. Corn,
Dustin J. Maly,
Douglas M. Fowler

Affiliations

John C. Rose: Department of Chemistry, University of Washington
Nicholas A. Popp: Department of Genome Sciences, University of Washington
Christopher D. Richardson: Innovative Genomics Initiative, University of California, Berkeley
Jason J. Stephany: Department of Genome Sciences, University of Washington
Julie Mathieu: Department of Comparative Medicine, Institute for Stem Cell and Regenerative Medicine, University of Washington
Cindy T. Wei: Department of Chemistry, University of Washington
Jacob E. Corn: Innovative Genomics Initiative, University of California, Berkeley
Dustin J. Maly: Department of Chemistry, University of Washington
Douglas M. Fowler: Department of Genome Sciences, University of Washington

DOI: https://doi.org/10.1038/s41467-020-16542-9
Journal volume & issue: Vol. 11, no. 1
pp. 1 – 11

Abstract

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Numerous strategies exist to limit the off-target activity of CRISPR-Cas9 nucleases. Here the authors co-administer truncated gRNAs that block both Cas9 and high-fidelity Cas9 variants from cleaving at off-target sites.

Published in Nature Communications

ISSN: 2041-1723 (Online)
Publisher: Nature Portfolio
Country of publisher: United Kingdom
LCC subjects: Science
Website: https://www.nature.com/ncomms/

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