High-dimensional super-resolution imaging reveals heterogeneity and dynamics of subcellular lipid membranes

Karl Zhanghao; Wenhui Liu; Meiqi Li; Zihan Wu; Xiao Wang; Xingye Chen; Chunyan Shan; Haoqian Wang; Xiaowei Chen; Qionghai Dai; Peng Xi; Dayong Jin

doi:10.1038/s41467-020-19747-0

Nature Communications (Nov 2020)

High-dimensional super-resolution imaging reveals heterogeneity and dynamics of subcellular lipid membranes

Karl Zhanghao,
Wenhui Liu,
Meiqi Li,
Zihan Wu,
Xiao Wang,
Xingye Chen,
Chunyan Shan,
Haoqian Wang,
Xiaowei Chen,
Qionghai Dai,
Peng Xi,
Dayong Jin

Affiliations

Karl Zhanghao: UTS-SUStech Joint Research Centre for Biomedical Materials & Devices, Department of Biomedical Engineering, College of Engineering, Southern University of Science and Technology
Wenhui Liu: Department of Automation, Tsinghua University
Meiqi Li: Department of Biomedical Engineering, College of Engineering, Peking University
Zihan Wu: UTS-SUStech Joint Research Centre for Biomedical Materials & Devices, Department of Biomedical Engineering, College of Engineering, Southern University of Science and Technology
Xiao Wang: State Key Laboratory of Biomembrane and Membrane Biotechnology, College of Life Sciences, Peking University
Xingye Chen: Department of Automation, Tsinghua University
Chunyan Shan: State Key Laboratory of Biomembrane and Membrane Biotechnology, College of Life Sciences, Peking University
Haoqian Wang: Department of Automation, Tsinghua University
Xiaowei Chen: State Key Laboratory of Biomembrane and Membrane Biotechnology, College of Life Sciences, Peking University
Qionghai Dai: Department of Automation, Tsinghua University
Peng Xi: UTS-SUStech Joint Research Centre for Biomedical Materials & Devices, Department of Biomedical Engineering, College of Engineering, Southern University of Science and Technology
Dayong Jin: UTS-SUStech Joint Research Centre for Biomedical Materials & Devices, Department of Biomedical Engineering, College of Engineering, Southern University of Science and Technology

DOI: https://doi.org/10.1038/s41467-020-19747-0
Journal volume & issue: Vol. 11, no. 1
pp. 1 – 10

Abstract

Read online

Lipid membranes are heterogeneous and dynamically regulated in cells. Here the authors report a Spectrum and Polarisation Optical Tomography (SPOT) method where they use Nile Red dye to resolve membrane morphology, polarity and phase in cells.

Published in Nature Communications

ISSN: 2041-1723 (Online)
Publisher: Nature Portfolio
Country of publisher: United Kingdom
LCC subjects: Science
Website: https://www.nature.com/ncomms/

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