International Journal of Nanomedicine (Feb 2020)

Aptamer-Conjugated Gold Nanoparticles Targeting Epidermal Growth Factor Receptor Variant III for the Treatment of Glioblastoma

  • Peng L,
  • Liang Y,
  • Zhong X,
  • Liang Z,
  • Tian Y,
  • Li S,
  • Liang J,
  • Wang R,
  • Zhong Y,
  • Shi Y,
  • Zhang X

Journal volume & issue
Vol. Volume 15
pp. 1363 – 1372

Abstract

Read online

Li Peng,1,2,* Yanling Liang,1,* Xinxin Zhong,1 Zhiman Liang,1,2 Yinghong Tian,3 Shuji Li,1 Jingxue Liang,4 Ransheng Wang,4 Yuqi Zhong,4 Yusheng Shi,5 Xingmei Zhang1 1Key Laboratory of Mental Health of the Ministry of Education, Guangdong-Hong Kong-Macao Greater Bay Area Center for Brain Science and Brain-Inspired Intelligence, Guangdong Province Key Laboratory of Psychiatric Disorders, Department of Neurobiology, School of Basic Medical Sciences, Southern Medical University, Guangzhou 510515, People’s Republic of China; 2The Fifth Affiliated Hospital, Southern Medical University, Guangzhou 510900, People’s Republic of China; 3Experiment Teaching & Administration Center, School of Basic Medical Sciences, Southern Medical University, Guangzhou 510515, People’s Republic of China; 4The First Affiliated Hospital, Southern Medical University, Guangzhou 510515, People’s Republic of China; 5Department of Radiation Oncology, Nanfang Hospital, Southern Medical University, Guangzhou 510515, People’s Republic of China*These authors contributed equally to this workCorrespondence: Xingmei ZhangKey Laboratory of Mental Health of the Ministry of Education, Guangdong-Hong Kong-Macao Greater Bay Area Center for Brain Science and Brain-Inspired Intelligence, Guangdong Province Key Laboratory of Psychiatric Disorders, Department of Neurobiology, School of Basic Medical Sciences, Southern Medical University, No. 1838 Guangzhou Avenue, Guangzhou 510515, People’s Republic of ChinaEmail [email protected] ShiDepartment of Radiation Oncology, Nanfang Hospital, Southern Medical University, No. 1838 Guangzhou Avenue, Guangzhou 510515, People’s Republic of ChinaEmail [email protected]: In this study, we constructed novel brain-targeting complexes (U2-AuNP) by conjugating aptamer U2 to the gold nanoparticle (AuNPs) surface as a promising option for GBM therapy.Materials and Methods: The properties of the U2-AuNP complexes were thoroughly characterized. Then, we detected the in vitro effects of U2-AuNP in U87-EGFRvIII cell lines and the in vivo antitumor effects of U2-AuNP in GBM-bearing mice. Furthermore, we explored the inhibition mechanism of U2-AuNP in U87-EGFRvIII cell lines.Results: We found that U2-AuNP inhibits the proliferation and invasion of U87-EGFRvIII cell lines and prolongs the survival time of GBM-bearing mice. We found that U2-AuNP can inhibit the EGFR-related pathway and prevent DNA damage repair in GBM cells.Conclusion: These results reveal the promising potential of U2-AuNP as a drug candidate for targeted therapy in GBM.Keywords: SELEX, EGFRvIII, GBM, therapy

Keywords