Isolating and Identification Some Types of Bacteria that Cause Otitis Media and Detecting their Resistance Towards Antibiotics

Abstract

One hundred ear swabs were collected from patients attending Hawija General Hospital, Azadi Teaching Hospital, and outpatient clinics in Kirkuk Governorate, for ages ranging between 2-45 years, During the period from October 27, 2022 to January 3, 2023. The results showed that 91 (91%) of samples have positive growth on MacConkey , mannitol and blood agar. While the remaining samples 9 (9%) had no growth. The samples that showed positive bacterial growth were identified as 139 isolates. The number of Gram positive isolates was 91, a rate of 65.5%, while the number of negative isolates was 48, a rate of 34.5%. The isolates were diagnosed using routine biochemical tests, and the diagnosis was confirmed by Vitek2 compact system device. The percentage of isolated bacterial species were Staphylococcus aureus by 34.53%, Staphylococcus epidermidis by 22.30%, Proteus mirabilis by 8.63%, Morganella morganii by 6.47%, Enterobacter sakazakii by 6.47%, Staphylococcus saprophyticus by 4.32%, E.coli by 3.60%, Klebsiella pneumonia by 3.60% And each of Kocuria kristinae, Proteus vulgaris, Comamonas testosterone by 2.88%, and Staphylococcus lentus by 1.44%.The results of the sensitivity test to ten types of antibiotics, namely Amikacin, Ceftazidime, Nitrofurantion, Imipenem, Azithromycin, Tetracycline, Cefotaxime, Tobramycin, Ofloxacin, Oxacillin, showed that all isolates were 100% resistant to the antibiotics Cetazidime and Cefotaxime, while they showed varying resistance to the rest of the antibiotics. Antibiotics: All isolates showed 100% sensitivity to Anti- imipenem.

Keywords

• otitis media infection
• pathogenic bacteria
• antibiotics