Development and Evaluation of a Duplex Lateral Flow Assay for the Detection and Differentiation between Rabbit Haemorrhagic Disease Virus <i>Lagovirus europaeus</i>/GI.1 and /GI.2
Alba Fresco-Taboada,
Mercedes Montón,
Istar Tapia,
Elena Soria,
Juan Bárcena,
Cécile Guillou-Cloarec,
Ghislaine Le Gall-Reculé,
Esther Blanco,
Paloma Rueda
Affiliations
Alba Fresco-Taboada
Eurofins Ingenasa S.A., 28037 Madrid, Spain
Mercedes Montón
Eurofins Ingenasa S.A., 28037 Madrid, Spain
Istar Tapia
Eurofins Ingenasa S.A., 28037 Madrid, Spain
Elena Soria
Eurofins Ingenasa S.A., 28037 Madrid, Spain
Juan Bárcena
Centro de Investigación en Sanidad Animal (CISA-INIA/CSIC), Valdeolmos, 28130 Madrid, Spain
Cécile Guillou-Cloarec
French Agency for Food, Environmental and Occupational Health & Safety (Anses), Avian and Rabbit Virology, Immunology and Parasitology Unit, Ploufragan-Plouzané-Niort Laboratory, 22440 Ploufragan, France
Ghislaine Le Gall-Reculé
French Agency for Food, Environmental and Occupational Health & Safety (Anses), Avian and Rabbit Virology, Immunology and Parasitology Unit, Ploufragan-Plouzané-Niort Laboratory, 22440 Ploufragan, France
Esther Blanco
Centro de Investigación en Sanidad Animal (CISA-INIA/CSIC), Valdeolmos, 28130 Madrid, Spain
Rabbit Haemorrhagic Disease Virus 2 (RHDV2, recently named Lagovirus europaeus/GI.2) was first reported in France in 2010 and has spread globally since then, replacing most of the circulating former RHDV (genotype GI.1) in many countries. The detection and differentiation of both genotypes is of crucial importance for the surveillance of the disease. In this article, a duplex lateral flow assay (LFA) for antigen detection is described and evaluated, providing the first description of a quick and easy-to-use test that allows for the simultaneous detection and differentiation of RHDV genotypes GI.1 and GI.2. A panel of GI.1- or GI.2-infected and non-infected rabbit liver samples and liver exudates (136 samples) was analysed, obtaining a total sensitivity of 94.4% and specificity of 100%. These data confirm that the developed duplex LFA can be used as a reliable diagnostic test for RHD surveillance, especially in farms and the field.
