Production of New Biopesticides from <i>Cymbopogon citratus</i> for the Control of Coffee Rust (<i>Hemileia vastatrix</i>) under Laboratory and Field Conditions
Luis Morales-Aranibar,
Francisca Elena Yucra Yucra,
Nivia Marisol Pilares Estrada,
Policarpo Quispe Flores,
Renatto Nicolino Motta Zevallos,
Juan Carlos Llanos Zegarra,
Uriel Palomino Trujillo,
Carlos Genaro Morales Aranibar,
Hebert Hernán Soto Gonzales,
Jorge González Aguilera,
Leandris Argentel-Martínez,
Sucleidi Nápoles Vinent
Affiliations
Luis Morales-Aranibar
National Intercultural University of Quillabamba, La Convenciòn, Cusco 08741, Peru
Francisca Elena Yucra Yucra
National Intercultural University of Quillabamba, La Convenciòn, Cusco 08741, Peru
Nivia Marisol Pilares Estrada
National Intercultural University of Quillabamba, La Convenciòn, Cusco 08741, Peru
Policarpo Quispe Flores
National Intercultural University of Quillabamba, La Convenciòn, Cusco 08741, Peru
Renatto Nicolino Motta Zevallos
National Intercultural University of Quillabamba, La Convenciòn, Cusco 08741, Peru
Juan Carlos Llanos Zegarra
National Intercultural University of Quillabamba, La Convenciòn, Cusco 08741, Peru
Uriel Palomino Trujillo
National Intercultural University of Quillabamba, La Convenciòn, Cusco 08741, Peru
Carlos Genaro Morales Aranibar
Jorge Basadre Grohmann National University (UNJBG), Tacna 23001, Peru
Hebert Hernán Soto Gonzales
National University of Moquegua (UNAM), Ilo 18601, Peru
Jorge González Aguilera
State University of Mato Grosso do Sul (UEMS), Cassilândia 79540-000, MS, Brazil
Leandris Argentel-Martínez
Tecnológico Nacional de México/Instituto Tecnológico del Valle del Yaqui, Bácum 85270, Sonora, Mexico
Sucleidi Nápoles Vinent
Universidad de Oriente, Santiago de Cuba 90600, Cuba
One of the diseases with the greatest economic impact on coffee cultivation worldwide and particularly in Peru is coffee rust (Hemileia vastatrix). The search for sustainable control methods as disease management strategies in coffee cultivation is necessary. The objective of this research was to determine the effectiveness of five biopesticides based on lemon verbena (Cymbopogon citratus) for the control of rust applied in laboratory and field conditions to allow the recovery of coffee (Coffea arabica L. var. typica) in La Convención, Cusco, Peru. Five biopesticides (oil, macerate, infusion, hydrolate and Biol) and four concentrations (0, 15, 20 and 25%) were evaluated. The biopesticides were evaluated under laboratory conditions (light and dark) at different concentrations. The design used was completely randomized in a factorial scheme. The biopesticides were incorporated into the culture medium and inoculated with 400 uredospores of rust, and the germination percentage was evaluated. Under field conditions, the biopesticides at the same concentrations were evaluated for 4 weeks after application. Under these field conditions, the incidence, severity and area under the disease progress curve (AUDPC) of selected plants with a natural degree of infection were evaluated. In the laboratory, the results showed that all biopesticides were effective in reducing the germination of rust uredospores to values Cymbopogon citratus oil is an excellent biopesticide that can be used to control coffee rust.
