油茶枯饼提取物主要生物活性成分及其对镉致 大鼠学习记忆损伤的影响Main bioactive compositions of oil-tea camellia seed cake extract and its effect on cadmium induced learning-memory impairment in rats

Abstract

研究油茶枯饼提取物的主要生物活性成分及其对镉致氧化应激和学习记忆损伤的保护作用。采用UPLC MS/MS分析油茶枯饼提取物的主要生物活性成分。将9周龄SD大鼠随机分为对照组（0.9% NaCl溶液灌胃+0.9%NaCl溶液腹腔注射）、染毒组（0.9%NaCl溶液灌胃+0.1%CdCl2 溶液腹腔注射）、干预组（1%油茶枯饼提取物溶液灌胃+0.1%CdCl2溶液腹腔注射），按每天灌胃，3 d 腹腔注射停1 d方式连续饲养31 d后，进行水迷宫试验，评估各组大鼠的学习记忆功能，计算脏器系数，并检测大鼠血清、肾脏、肝脏和大脑中谷胱甘肽过氧化物酶（GSH-Px）活性、超氧化物歧化酶（SOD）活性和丙二醛（MDA）含量。结果表明：油茶枯饼提取物主要含15种生物活性成分；与对照组相比，染毒组大鼠的肾脏和肝脏的脏器系数极显著增加（P<0.001），血清GSH-Px活性极显著降低（P<0.001），肝脏SOD活性显著降低（P<0.05），大鼠寻找平台的潜伏期显著延长（P＜005）；使用油茶枯饼提取物干预后，这些指标均得到一定程度的恢复。因此，油茶枯饼提取物可以在一定程度上抑制镉诱导的氧化应激和认知功能障碍。 The main bioactive compositions of oil-tea camellia seed cake extract and its effect on protecting organs against oxidative stress and ameliorating the learning-memory impairment induced by cadmium were explored. UPLC MS/MS was used to analyze the main bioactive compositions. Nine-week old SD rats were randomly divided into control group (0.9% NaCl administration+0.9% NaCl intraperitoneal injection), exposure group (0.9% NaCl administration+0.1% CdCl2 intraperitoneal injection) and intervention group (1% oil-tea camellia seed cake extract administration+0.1% CdCl2 intraperitoneal injection). And the rate were fed by daily gavage and 3 d intraperitoneal injection with 1 d off for 31 d, then the Morris water maze test was used to assess the learning-memory function of the rats in the different groups, and the organ coefficients were calculated, and indicators of oxidation, including glutathione peroxidase (GSH-Px) activity, superoxide dismutase (SOD) activity and malondialdehyde (MDA) content of serum, kidney, liver and brain were determined. The results showed that oil-tea camellia seed cake extract contained 15 kinds of bioactive components. Compared with the control group, the organ coefficient of kidney and liver in the exposure group rats increased very significantly (P<0.001), the activities of serum GSH-Px and liver SOD decreased very significantly (P<0.001) and significantly (P<0.05) respectively, and the latency of looking for platform was prolonged significantly (P<0.05). After the intervention of oil-tea camellia seed cake extract, these indexes were restored to a certain extent. Therefore, oil-tea camellia seed cake extract could inhibit cadmium induced oxidative stress and learning-memory impairment to a certain extent.

Keywords

• 镉；油茶枯饼提取物；氧化应激；学习记忆
• cadmium; oil-tea camellia seed cake extract; oxidative stress; learning-memory