Revista da Sociedade Brasileira de Medicina Tropical ()

Brazil’s first free-mating laboratory colony of Nyssorhynchus darlingi

  • Maisa da Silva Araujo,
  • Alice Oliveira Andrade,
  • Najara Akira Costa dos Santos,
  • Dhélio Batista Pereira,
  • Glaucilene da Silva Costa,
  • Paula Frassinetti Medeiros de Paulo,
  • Carlos Tong Rios,
  • Marta Moreno,
  • Luiz Hidelbrando Pereira-da-Silva,
  • Jansen Fernandes de Medeiros

DOI
https://doi.org/10.1590/0037-8682-0159-2019
Journal volume & issue
Vol. 52

Abstract

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Abstract INTRODUCTION: The lack of highly-productive Nyssorhynchus darlingi laboratory colonies limits some studies. We report the first well-established laboratory colony of Ny. darlingi in Brazil. METHODS: Mosquitoes were collected from Porto Velho and were reared at the Laboratory of Fiocruz/RO. After induced mating by light stimulation in the F1 to F6, the subsequent generations were free mating. Larvae were reared in distilled water and fed daily until pupation. RESULTS: In 11 generations, the colony produced a high number of pupae after the F5 generation. CONCLUSIONS: These results demonstrate the potential for permanently establishing Ny. darlingi colonies for research purposes in Brazil.

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