Nature Communications (Nov 2024)
A single NLR gene confers resistance to leaf and stripe rust in wheat
- Davinder Sharma,
- Raz Avni,
- Juan Gutierrez-Gonzalez,
- Rakesh Kumar,
- Hanan Sela,
- Manas Ranjan Prusty,
- Arava Shatil-Cohen,
- István Molnár,
- Kateřina Holušová,
- Mahmoud Said,
- Jaroslav Doležel,
- Eitan Millet,
- Sofia Khazan-Kost,
- Udi Landau,
- Gerit Bethke,
- Or Sharon,
- Smadar Ezrati,
- Moshe Ronen,
- Oxana Maatuk,
- Tamar Eilam,
- Jacob Manisterski,
- Pnina Ben-Yehuda,
- Yehoshua Anikster,
- Oadi Matny,
- Brian J. Steffenson,
- Martin Mascher,
- Helen J. Brabham,
- Matthew J. Moscou,
- Yong Liang,
- Guotai Yu,
- Brande B. H. Wulff,
- Gary Muehlbauer,
- Anna Minz-Dub,
- Amir Sharon
Affiliations
- Davinder Sharma
- The Institute for Cereal Crops Research, Tel Aviv University
- Raz Avni
- The Institute for Cereal Crops Research, Tel Aviv University
- Juan Gutierrez-Gonzalez
- Department of Agronomy and Plant Genetics, University of Minnesota
- Rakesh Kumar
- The Institute for Cereal Crops Research, Tel Aviv University
- Hanan Sela
- The Institute for Cereal Crops Research, Tel Aviv University
- Manas Ranjan Prusty
- The Institute for Cereal Crops Research, Tel Aviv University
- Arava Shatil-Cohen
- The Institute for Cereal Crops Research, Tel Aviv University
- István Molnár
- Institute of Experimental Botany of the Czech Academy of Sciences, Centre of Plant Structural and Functional Genomics
- Kateřina Holušová
- Institute of Experimental Botany of the Czech Academy of Sciences, Centre of Plant Structural and Functional Genomics
- Mahmoud Said
- Institute of Experimental Botany of the Czech Academy of Sciences, Centre of Plant Structural and Functional Genomics
- Jaroslav Doležel
- Institute of Experimental Botany of the Czech Academy of Sciences, Centre of Plant Structural and Functional Genomics
- Eitan Millet
- The Institute for Cereal Crops Research, Tel Aviv University
- Sofia Khazan-Kost
- The Institute for Cereal Crops Research, Tel Aviv University
- Udi Landau
- The Institute for Cereal Crops Research, Tel Aviv University
- Gerit Bethke
- Department of Agronomy and Plant Genetics, University of Minnesota
- Or Sharon
- The Institute for Cereal Crops Research, Tel Aviv University
- Smadar Ezrati
- The Institute for Cereal Crops Research, Tel Aviv University
- Moshe Ronen
- The Institute for Cereal Crops Research, Tel Aviv University
- Oxana Maatuk
- The Institute for Cereal Crops Research, Tel Aviv University
- Tamar Eilam
- The Institute for Cereal Crops Research, Tel Aviv University
- Jacob Manisterski
- The Institute for Cereal Crops Research, Tel Aviv University
- Pnina Ben-Yehuda
- The Institute for Cereal Crops Research, Tel Aviv University
- Yehoshua Anikster
- The Institute for Cereal Crops Research, Tel Aviv University
- Oadi Matny
- Department of Plant Pathology, University of Minnesota
- Brian J. Steffenson
- Department of Plant Pathology, University of Minnesota
- Martin Mascher
- Leibniz Institute of Plant Genetics and Crop Plant Research (IPK) Gatersleben
- Helen J. Brabham
- The Sainsbury Laboratory, Norwich Research Park
- Matthew J. Moscou
- USDA-ARS, Cereal Disease Laboratory, University of Minnesota
- Yong Liang
- School of Plant Sciences and Food Security, Tel Aviv University
- Guotai Yu
- John Innes Centre, Norwich Research Park
- Brande B. H. Wulff
- John Innes Centre, Norwich Research Park
- Gary Muehlbauer
- Department of Agronomy and Plant Genetics, University of Minnesota
- Anna Minz-Dub
- The Institute for Cereal Crops Research, Tel Aviv University
- Amir Sharon
- The Institute for Cereal Crops Research, Tel Aviv University
- DOI
- https://doi.org/10.1038/s41467-024-54068-6
- Journal volume & issue
-
Vol. 15,
no. 1
pp. 1 – 16
Abstract
Abstract Nucleotide-binding leucine-rich repeat (NLR) disease resistance genes typically confer resistance against races of a single pathogen. Here, we report that Yr87/Lr85, an NLR gene from Aegilops sharonensis and Aegilops longissima, confers resistance against both P. striiformis tritici (Pst) and Puccinia triticina (Pt) that cause stripe and leaf rust, respectively. Yr87/Lr85 confers resistance against Pst and Pt in wheat introgression as well as transgenic lines. Comparative analysis of Yr87/Lr85 and the cloned Triticeae NLR disease resistance genes shows that Yr87/Lr85 contains two distinct LRR domains and that the gene is only found in Ae. sharonensis and Ae. longissima. Allele mining and phylogenetic analysis indicate multiple events of Yr87/Lr85 gene flow between the two species and presence/absence variation explaining the majority of resistance to wheat leaf rust in both species. The confinement of Yr87/Lr85 to Ae. sharonensis and Ae. longissima and the resistance in wheat against Pst and Pt highlight the potential of these species as valuable sources of disease resistance genes for wheat improvement.
