The antibacterial effects of chitosan nanoparticles from Scylla sp. on acne-related bacteria: Staphylococcus aureus and Staphylococcus epidermidis

Abstract

One of the many advantages of chitosan obtained from the shells of mud crabs is that it acts as an antibiotic against acne. This study set out to determine chitosan’s characteristic properties that were taken from Scylla sp shells, the nanoparticle’s dimension, and the optimal chitosan nanoparticle concentration to stop the growth of acne-causing Staphylococcus aureus and Staphylococcus epidermidis. A descriptive approach is used in this research methodology to evaluate the chitosan’s quality and antibacterial efficacy. Chitosan nanoparticles at varying concentrations (0.20%, 0.25%, 0.50%, 1%, and 1.25%) are included as the only independent factor, and changes are examined within a 95% confidence interval. The positive control is ampicillin, while the negative control is acetic acid. A Particle Size Analyzer (PSA) was used to analyze the chitosan nanoparticles’ dimensions, and the results showed that they were 47.04 nm. At different doses, chitosan nanoparticles’ inhibitory effect on the growth of S. aureus and S. epidermidis was assessed. Both S. aureus and S. epidermidis growth were effectively inhibited at a concentration of 0.50 percent. The chitosan nanoparticle activity in this investigation fell within the moderate to strong range.