Nature Communications (Oct 2022)
Cell-specific bioorthogonal tagging of glycoproteins
- Anna Cioce,
- Beatriz Calle,
- Tatiana Rizou,
- Sarah C. Lowery,
- Victoria L. Bridgeman,
- Keira E. Mahoney,
- Andrea Marchesi,
- Ganka Bineva-Todd,
- Helen Flynn,
- Zhen Li,
- Omur Y. Tastan,
- Chloe Roustan,
- Pablo Soro-Barrio,
- Mahmoud-Reza Rafiee,
- Acely Garza-Garcia,
- Aristotelis Antonopoulos,
- Thomas M. Wood,
- Tessa Keenan,
- Peter Both,
- Kun Huang,
- Fabio Parmeggian,
- Ambrosius P. Snijders,
- Mark Skehel,
- Svend Kjær,
- Martin A. Fascione,
- Carolyn R. Bertozzi,
- Stuart M. Haslam,
- Sabine L. Flitsch,
- Stacy A. Malaker,
- Ilaria Malanchi,
- Benjamin Schumann
Affiliations
- Anna Cioce
- Department of Chemistry, Imperial College London
- Beatriz Calle
- Department of Chemistry, Imperial College London
- Tatiana Rizou
- Tumour-Host Interaction Laboratory, The Francis Crick Institute
- Sarah C. Lowery
- Department of Chemistry, Yale University
- Victoria L. Bridgeman
- Tumour-Host Interaction Laboratory, The Francis Crick Institute
- Keira E. Mahoney
- Department of Chemistry, Yale University
- Andrea Marchesi
- Department of Chemistry, Imperial College London
- Ganka Bineva-Todd
- Chemical Glycobiology Laboratory, The Francis Crick Institute
- Helen Flynn
- Proteomics Science Technology Platform, The Francis Crick Institute
- Zhen Li
- Department of Chemistry, Imperial College London
- Omur Y. Tastan
- Chemical Glycobiology Laboratory, The Francis Crick Institute
- Chloe Roustan
- Structural Biology Science Technology Platform, The Francis Crick Institute
- Pablo Soro-Barrio
- Bioinformatics & Biostatistics Science Technology Platform, The Francis Crick Institute
- Mahmoud-Reza Rafiee
- RNA Networks Laboratory, The Francis Crick Institute
- Acely Garza-Garcia
- Mycobacterial Metabolism and Antibiotic Research Laboratory, The Francis Crick Institute
- Aristotelis Antonopoulos
- Department of Life Sciences, Imperial College London
- Thomas M. Wood
- Sarafan ChEM-H, Department of Chemistry and Howard Hughes Medical Institute, Stanford University
- Tessa Keenan
- Department of Chemistry, University of York
- Peter Both
- School of Chemistry & Institute of Biotechnology, The University of Manchester
- Kun Huang
- School of Chemistry & Institute of Biotechnology, The University of Manchester
- Fabio Parmeggian
- School of Chemistry & Institute of Biotechnology, The University of Manchester
- Ambrosius P. Snijders
- Proteomics Science Technology Platform, The Francis Crick Institute
- Mark Skehel
- Proteomics Science Technology Platform, The Francis Crick Institute
- Svend Kjær
- Structural Biology Science Technology Platform, The Francis Crick Institute
- Martin A. Fascione
- Department of Chemistry, University of York
- Carolyn R. Bertozzi
- Sarafan ChEM-H, Department of Chemistry and Howard Hughes Medical Institute, Stanford University
- Stuart M. Haslam
- Department of Life Sciences, Imperial College London
- Sabine L. Flitsch
- School of Chemistry & Institute of Biotechnology, The University of Manchester
- Stacy A. Malaker
- Department of Chemistry, Yale University
- Ilaria Malanchi
- Tumour-Host Interaction Laboratory, The Francis Crick Institute
- Benjamin Schumann
- Department of Chemistry, Imperial College London
- DOI
- https://doi.org/10.1038/s41467-022-33854-0
- Journal volume & issue
-
Vol. 13,
no. 1
pp. 1 – 18
Abstract
Changes in glycoprotein expression are correlates of disease, but secreted glycoproteins cannot be accurately traced to their cell line of origin. Here, the authors develop a strategy to chemically tag and profile glycoproteins in a cell line-specific manner in co-culture systems and in vivo.
